Waf1/Cip1/CDKN1A p21 Antibody (F-5): sc-6246

p21 Waf1/Cip1 Antibody (F-5) is a mouse monoclonal IgG2b kappa light chain antibody that targets the full-length p21 Waf1/Cip1 protein (amino acids 1–159) of mouse origin, and is recommended for use in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHC), and flow cytometry in mouse, rat, and human samples. p21 Waf1/Cip1 monoclonal antibody (F-5) is available in both non-conjugated and various conjugated forms, such as agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates, providing flexibility for diverse experimental needs. p21 Waf1/Cip1, also known as cyclin-dependent kinase inhibitor 1 (CDKN1A) or CDK-interacting protein 1 (CIP1), plays a pivotal role in cell cycle regulation by inhibiting cyclin-dependent kinases (CDKs), thereby controlling the transition from the G1 to the S phase of the cell cycle. This inhibition is crucial for maintaining genomic stability, as it allows for DNA repair before cell division proceeds. p21 expression is primarily regulated by the tumor suppressor protein p53 in response to DNA damage, linking it to cellular stress responses and tumor suppression mechanisms. Additionally, p21 interacts with proliferating cell nuclear antigen (PCNA), influencing DNA replication and repair processes. Given its integral role in cell cycle control and its association with cancer development, p21 is a significant focus in cancer research and therapeutic strategies. p21 Waf1/Cip1 monoclonal antibody (F-5) enables researchers to investigate the expression and function of this critical protein across various biological contexts.

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Waf1/Cip1/CDKN1A p21 Antibody (F-5) References:

1. Loss of Notch1-dependent p21(Waf1/Cip1) expression influences the Notch1 outcome in tumorigenesis.  |  Cialfi, S., et al. 2014. Cell Cycle. 13: 2046-55. PMID: 24801890
2. P21Waf1/Cip1 depletion promotes dexamethasone-induced apoptosis in osteoblastic MC3T3-E1 cells by inhibiting the Nrf2/HO-1 pathway.  |  Han, D., et al. 2018. Arch Toxicol. 92: 679-692. PMID: 28940008
3. Absence of p21(WAF1/CIP1/SDI1) protects against osteopenia and minimizes bone loss after ovariectomy in a mouse model.  |  Premnath, P., et al. 2019. PLoS One. 14: e0215018. PMID: 30970032
4. Essential role of p21Waf1/Cip1 in the modulation of post-traumatic hippocampal Neural Stem Cells response.  |  Chiani, F., et al. 2024. Stem Cell Res Ther. 15: 197. PMID: 38971774
5. WAF1/CIP1 is induced in p53-mediated G1 arrest and apoptosis.  |  el-Deiry, WS., et al. 1994. Cancer Res. 54: 1169-74. PMID: 8118801
6. The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases.  |  Harper, JW., et al. 1993. Cell. 75: 805-16. PMID: 8242751
7. WAF1, a potential mediator of p53 tumor suppression.  |  el-Deiry, WS., et al. 1993. Cell. 75: 817-25. PMID: 8242752
8. Braking the cycle.  |  Hunter, T. 1993. Cell. 75: 839-41. PMID: 8252620
9. p21 is a universal inhibitor of cyclin kinases.  |  Xiong, Y., et al. 1993. Nature. 366: 701-4. PMID: 8259214
10. Inhibition of CDK2 activity in vivo by an associated 20K regulatory subunit.  |  Gu, Y., et al. 1993. Nature. 366: 707-10. PMID: 8259216
11. Direct binding of cyclin D to the retinoblastoma gene product (pRb) and pRb phosphorylation by the cyclin D-dependent kinase CDK4.  |  Kato, J., et al. 1993. Genes Dev. 7: 331-42. PMID: 8449399
12. Mammalian G1 cyclins.  |  Sherr, CJ. 1993. Cell. 73: 1059-65. PMID: 8513492

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Waf1/Cip1/CDKN1A p21 Antibody (F-5) has 1,895 citations in a variety of scientific publications.

Primary antibodies like Anti-Waf1/Cip1/CDKN1A p21 Antibody (F-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Waf1/Cip1/CDKN1A p21 Antibody (F-5) Specifications

Alternate Product Name: p21 Waf1/Cip1

Product Clone Name: F-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-159

Epitope Target: p21 Waf1/Cip1

Epitope Species: mouse

Product Reactivity/Detection: p21 Waf1/Cip1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1,895 citations in various scientific publications

Available Conjugates/Forms for Waf1/Cip1/CDKN1A p21 Antibody (F-5): AC: Agarose, AF405: Alexa Fluor^® 405, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, B: Biotin, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, TRITC: Rhodamine