p-Vav Antibody (9.Tyr 174): sc-135788

p-Vav Antibody (9.Tyr 174) is a mouse monoclonal IgG2b kappa light chain antibody that detects the Tyr 174 phosphorylated Vav protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). anti-p-Vav antibody (9.Tyr 174) is available in both conjugated and non-conjugated forms, providing flexibility for various experimental needs. Vav proteins serve as guanine nucleotide exchange factors for Rho family GTPases, playing a crucial role in activating signaling pathways that lead to actin cytoskeletal rearrangements and transcriptional changes, which are essential for processes such as cell migration and proliferation. Vav protein′s structure includes several protein-binding domains that facilitate the connection between cell surface receptors and downstream signaling proteins, thereby influencing cellular responses to external stimuli. Notably, Vav3, a member of the Vav family, interacts with receptor protein tyrosine kinases and exhibits a unique expression profile across different tissues, distinguishing Vav3 from Vav and Vav2. This interaction mediates receptor tyrosine kinase signaling and regulates GTPase activity, with both native and mutant forms of Vav3 capable of altering cell morphology and potentially inducing cell transformation. Phosphorylation of Tyrosine 174 is a key regulatory event that occurs upon stimulation of mitogenic and antigenic receptors, a modification that is conserved among Vav family members and underscores Vav proteins′ importance in cellular signaling pathways.

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p-Vav Antibody (9.Tyr 174) References:

1. Biological and regulatory properties of Vav-3, a new member of the Vav family of oncoproteins.  |  Movilla, N. and Bustelo, XR. 1999. Mol Cell Biol. 19: 7870-85. PMID: 10523675
2. Tyrosine phosphorylation mediates both activation and downmodulation of the biological activity of Vav.  |  López-Lago, M., et al. 2000. Mol Cell Biol. 20: 1678-91. PMID: 10669745
3. Major transcript variants of VAV3, a new member of the VAV family of guanine nucleotide exchange factors.  |  Trenkle, T., et al. 2000. Gene. 245: 139-49. PMID: 10713454
4. Specific subdomains of Vav differentially affect T cell and NK cell activation.  |  Billadeau, DD., et al. 2000. J Immunol. 164: 3971-81. PMID: 10754287
5. Vav family proteins couple to diverse cell surface receptors.  |  Moores, SL., et al. 2000. Mol Cell Biol. 20: 6364-73. PMID: 10938113
6. Vav3 mediates receptor protein tyrosine kinase signaling, regulates GTPase activity, modulates cell morphology, and induces cell transformation.  |  Zeng, L., et al. 2000. Mol Cell Biol. 20: 9212-24. PMID: 11094073
7. Requirement of tyrosine-phosphorylated Vav for morphological differentiation of all-trans-retinoic acid-treated HL-60 cells.  |  Bertagnolo, V., et al. 2001. Cell Growth Differ. 12: 193-200. PMID: 11331248
8. Vav mediates Ras stimulation by direct activation of the GDP/GTP exchange factor Ras GRP1.  |  Caloca, MJ., et al. 2003. EMBO J. 22: 3326-36. PMID: 12839994
9. Association of PI 3-K with tyrosine phosphorylated Vav is essential for its activity in neutrophil-like maturation of myeloid cells.  |  Bertagnolo, V., et al. 2004. Cell Signal. 16: 423-33. PMID: 14709332
10. Vav proteins do not influence dengue virus replication but are associated with induction of phospho-ERK, IL-6, and viperin mRNA following DENV infection in vitro.  |  Cowell, E., et al. 2024. Microbiol Spectr. 12: e0239123. PMID: 38054722

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-Vav Antibody (9.Tyr 174) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-p-Vav Antibody (9.Tyr 174) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-Vav Antibody (9.Tyr 174) Specifications

Alternate Product Name: Vav

Product Clone Name: 9.Tyr 174

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: Tyr 174

Epitope Target: Vav

Epitope Species: human

Product Reactivity/Detection: Tyr 174 phosphorylated Vav

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for p-Vav Antibody (9.Tyr 174): AC: Agarose, HRP: Horseradish Peroxidase

The preferred Vav antibody is Vav Antibody (D-7): sc-8039.