Vav Antibody (D-7): sc-8039

Vav Antibody (D-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects the Vav protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Vav antibody (D-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Vav protein, also known as Vav1 or p95Vav, plays a crucial role in signal transduction, particularly in hematopoietic cells, where Vav1 is exclusively expressed. Vav protein is characterized by its unique structure, which includes an N-terminal helix-loop-helix domain and a leucine zipper motif, both of which are essential for function in mediating interactions with other proteins and facilitating downstream signaling pathways. The presence of an SH2 domain further suggests that Vav1 acts as a substrate for tyrosine kinases, linking receptor activation to cellular responses. The oncogenic potential of Vav protein was first identified through gene transfer assays, highlighting Vav1′s importance in cancer biology. Understanding the structural features and functional implications of Vav protein enables researchers to better explore Vav1′s role in hematopoietic signaling and potential as a therapeutic target in various diseases.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Vav Antibody (D-7) References:

1. Interferon-alpha signaling promotes nucleus-to-cytoplasmic redistribution of p95Vav, and formation of a multisubunit complex involving Vav, Ku80, and Tyk2.  |  Adam, L., et al. 2000. Biochem Biophys Res Commun. 267: 692-6. PMID: 10673353
2. Product of vav proto-oncogene defines a new class of tyrosine protein kinase substrates.  |  Bustelo, XR., et al. 1992. Nature. 356: 68-71. PMID: 1311423
3. Tyrosine phosphorylation of vav proto-oncogene product containing SH2 domain and transcription factor motifs.  |  Margolis, B., et al. 1992. Nature. 356: 71-4. PMID: 1531699
4. Vav-family proteins in T-cell signalling.  |  Tybulewicz, VL. 2005. Curr Opin Immunol. 17: 267-74. PMID: 15886116
5. Loss of the amino-terminal helix-loop-helix domain of the vav proto-oncogene activates its transforming potential.  |  Katzav, S., et al. 1991. Mol Cell Biol. 11: 1912-20. PMID: 2005887
6. Mechanism of activation of the vav protooncogene.  |  Coppola, J., et al. 1991. Cell Growth Differ. 2: 95-105. PMID: 2069873
7. Signal transduction by receptors with tyrosine kinase activity.  |  Ullrich, A. and Schlessinger, J. 1990. Cell. 61: 203-12. PMID: 2158859
8. vav, a novel human oncogene derived from a locus ubiquitously expressed in hematopoietic cells.  |  Katzav, S., et al. 1989. EMBO J. 8: 2283-90. PMID: 2477241
9. Vav family exchange factors: an integrated regulatory and functional view.  |  Bustelo, XR. 2014. Small GTPases. 5: 9. PMID: 25483299
10. p95vav associates with the nuclear protein Ku-70.  |  Romero, F., et al. 1996. Mol Cell Biol. 16: 37-44. PMID: 8524317
11. The VAV family of signal transduction molecules.  |  Bustelo, XR. 1996. Crit Rev Oncog. 7: 65-88. PMID: 9109498
12. Structure and function of vav.  |  Romero, F. and Fischer, S. 1996. Cell Signal. 8: 545-53. PMID: 9115846

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Vav Antibody (D-7) has 26 citations in a variety of scientific publications.

Primary antibodies like Anti-Vav Antibody (D-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Vav Antibody (D-7) Specifications

Alternate Product Name: Vav

Product Clone Name: D-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 110-320

Epitope Target: Vav p95

Epitope Species: human

Product Reactivity/Detection: Vav p95

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 26 citations in various scientific publications

Available Conjugates/Forms for Vav Antibody (D-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin