p-Stat1 Antibody (pY701.4A): sc-136229

p-Stat1 Antibody (pY701.4A) is a mouse monoclonal IgG2a kappa light chain antibody that detects Tyr 701 phosphorylated Stat1α and Stat1β from mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). p-Stat1 (pY701.4A) antibody is available in a non-conjugated form, providing flexibility for various experimental needs. Stat1 plays a crucial role in immune response and cellular signaling pathways, particularly in mediating interferon and cytokine effects. Upon activation, Stat1 undergoes phosphorylation at Tyr 701, essential for dimerization and subsequent nuclear translocation, where Stat1 regulates gene expression involved in immune responses and cell growth. Phosphorylation of Stat1 is primarily mediated by Janus kinases (JAKs), activated by membrane receptor signaling from ligands such as interferons and growth factors. This process enables cells to respond effectively to pathogens and other stressors. Stat1 also interacts with other proteins in the signaling cascade, including Stat2, to form the ISGF3 transcription factor complex, critical for transcriptional response to type I interferons. anti-Stat1 antibody (pY701.4A) specifically detects phosphorylated Stat1, making this antibody an invaluable tool for researchers studying immune signaling pathways and their implications in various diseases.

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p-Stat1 Antibody (pY701.4A) References:

1. Serine-phosphorylated STAT1 is a prosurvival factor in Wilms' tumor pathogenesis.  |  Timofeeva, OA., et al. 2006. Oncogene. 25: 7555-64. PMID: 16799645
2. Molecular basis for the recognition of phosphorylated STAT1 by importin alpha5.  |  Nardozzi, J., et al. 2010. J Mol Biol. 402: 83-100. PMID: 20643137
3. Importance of serine727 phosphorylated STAT1 in IFNγ-induced signaling and apoptosis of human salivary gland cells.  |  Tsuboi, H., et al. 2011. Int J Rheum Dis. 14: 86-91. PMID: 21303487
4. Inhibition of phosphorylated-STAT1 nuclear translocation and antiviral protein expression in human brain vascular adventitial fibroblasts infected with varicella-zoster virus.  |  Nagel, MA., et al. 2014. J Virol. 88: 11634-7. PMID: 25056900
5. Ebola virus VP24 targets a unique NLS binding site on karyopherin alpha 5 to selectively compete with nuclear import of phosphorylated STAT1.  |  Xu, W., et al. 2014. Cell Host Microbe. 16: 187-200. PMID: 25121748
6. Upregulation of the Suppressors of Cytokine Signaling 1 and 3 Is Associated with Arrest of Phosphorylated-STAT1 Nuclear Importation and Reduced Innate Response in Denguevirus-Infected Macrophages.  |  Estrada-Jiménez, T., et al. 2016. Viral Immunol. 29: 95-104. PMID: 26709547
7. The involvement of protein TNFSF18 in promoting p-STAT1 phosphorylation to induce coronary microcirculation disturbance in atherosclerotic mouse model.  |  Gao, J., et al. 2021. Drug Dev Res. 82: 115-122. PMID: 32820830
8. Mucosal p-STAT1/3 correlates with histologic disease activity in Crohn's disease and is responsive to filgotinib.  |  Reinisch, W., et al. 2023. Tissue Barriers. 11: 2088961. PMID: 35762272
9. Tyrosine-phosphorylated Stat1 and Stat2 plus a 48-kDa protein all contact DNA in forming interferon-stimulated-gene factor 3.  |  Qureshi, SA., et al. 1995. Proc Natl Acad Sci U S A. 92: 3829-33. PMID: 7537377
10. The rapid inactivation of nuclear tyrosine phosphorylated Stat1 depends upon a protein tyrosine phosphatase.  |  Haspel, RL., et al. 1996. EMBO J. 15: 6262-8. PMID: 8947049

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-Stat1 Antibody (pY701.4A) has 35 citations in a variety of scientific publications.

Primary antibodies like Anti-p-Stat1 Antibody (pY701.4A) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-Stat1 Antibody (pY701.4A) Specifications

Alternate Product Name: STAT1

Product Clone Name: pY701.4A

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: Tyr 701

Epitope Target: Stat1

Epitope Species: human

Product Reactivity/Detection: Tyr 701 phosphorylated Stat1α and Stat1β

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 35 citations in various scientific publications

Available Conjugates/Forms for p-Stat1 Antibody (pY701.4A): N/A

The preferred Stat1 antibody is Stat1 Antibody (C-136): sc-464.