P-Selectin Antibody (1 E 3) recommended for detection of P-Selectin of human origin by WB, IP, IF, IHC(P) and FCM

P-Selectin Antibody (1 E 3): sc-19672

P-Selectin Antibody (1 E 3) is rated 4.4 out of 5 by 7.
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Datasheets

    • P-Selectin Antibody (1 E 3) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against fixed thrombin-activated human platelets
    • P-Selectin Antibody (1 E 3) is recommended for detection of P-Selectin of human origin by WB, IP, IF, IHC(P) and FCM
    • Anti-P-Selectin Antibody (1 E 3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of P-Selectin (1 E 3): sc-19672.
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Regarding my question about another antibody, I'm not looking for a secondary antibody, but for another P-selectin antibody that could be used as the detector in ELISA that would bind at a spatially distinct epitope. Can you recommend one? Thanks

Asked by: tracey01
Thank you for your question. We do not characterize pairs of monoclonal antibodies for use in sandwich ELISA. Many of our antibodies are recommended as detection antibodies in direct or indirect ELISA, however we cannot recommend a matching capture antibody. Please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Technical Service
Date published: 2017-04-20

Do you have any data on performance in ELISA? If the antibody is known or predicted to work in ELISA, can you recommend a second antibody to use in the ELISA pair? Thank you

Asked by: tracey01
Thank you for your question. We have not yet tested this specific mouse monoclonal, P-Selectin Antibody (1 E 3): sc-19672, for ELISA so we do not have any specific recommendations we can offer at this time for this research application. However, the antibody has been tested in Western blot, immunohistochemistry and flow cytometry so we believe it should also work for ELISA. P-Selectin Antibody (1 E 3) is a mouse monoclonal IgG2a, so for a secondary antibody you would want to use an anti-mouse IgG secondary antibody conjugated to either alkaline phosphatase (AP) or horseradish peroxidase (HRP), depending on what detection method being used. We would recommend our mouse IgG binding protein conjugated to HRP, m-IgG kappa BP-HRP: sc-516102. A link to the product description is provided below for your reference. https://www.scbt.com/scbt/product/m-igg-kappa-bp-hrp
Answered by: Technical Support
Date published: 2017-04-18

Can P-Selectin (1 E 3): sc-19672 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, P-Selectin (1 E 3): sc-19672 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24

Dear Mr. or Ms. We would like to know if this CD62P antibody cross reacts with canine platelets.  I look forward to hearing from you.

Asked by: Peres Badial
Thank you for your question. We do not know whether or not this antibody will detect canine. P-Selectin Antibody (1 E 3) is a mouse monoclonal raised against fixed thrombin-activated human platelets. The exact epitope recognized by this antibody has not been determined so we are unable to try to predict cross-reactivity based on sequence analysis. This would have to be determined experimentally.
Answered by: Technical Support
Date published: 2017-02-08
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Rated 5 out of 5 by from Strong WB signal obtained in human platelet Strong WB signal obtained in human platelet extract. -SCBT QC
Date published: 2015-05-18
Rated 4 out of 5 by from Robust immunoperoxidase membrane staining Robust immunoperoxidase membrane staining in formalin-fixed human platelets and white blood cells. -SCBT QC
Date published: 2015-03-23
Rated 4 out of 5 by from WB: Worked well in human SV40 cells WB: Worked well in human SV40 cells. -SCBT Publication Review
Date published: 2015-01-26
Rated 5 out of 5 by from Published IF data with HUVECs Published IF data with HUVECs -SCBT Publication Review
Date published: 2015-01-24
Rated 5 out of 5 by from Good quality antibody for human paraffin samples Good quality antibody for human paraffin samples. Published in baboon artery (ductus arteriorus). -SCBT Publication Review
Date published: 2015-01-03
Rated 4 out of 5 by from Wonderful FCM data of human platelets Wonderful FCM data of human platelets. -SCBT QC
Date published: 2014-05-05
Rated 4 out of 5 by from Nice indirect FCM data of human platelets Nice indirect FCM data of human platelets stained with P-Selectin . -SCBT QC
Date published: 2014-05-05
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