P-Selectin Antibody (CTB201) recommended for detection of P-Selectin of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM

P-Selectin Antibody (CTB201): sc-8419

P-Selectin Antibody (CTB201) is rated 4.8 out of 5 by 16.
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Datasheets

    • Anti-P-Selectin Antibody (CTB201) is a mouse monoclonal IgG1 (kappa light chain) P-Selectin antibody provided at 200 µg/ml
    • raised against P-Selectin of human origin
    • Anti-P-Selectin Antibody (CTB201) is recommended for detection of P-Selectin of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
    • P-Selectin Antibody (CTB201) is available conjugated to agarose (sc-8419 AC), 500 µg/ml, 25% agarose, for IP; and to either phycoerythrin (sc-8419 PE) or FITC (sc-8419 FITC), 200 µg/ml, for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to either PerCP or PerCP-Cy5.5 for IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of P-Selectin (CTB201): sc-8419.
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Can you maybe provide any information regarding the isoelectric point of this antibody or guide me in the right direction, please?

Asked by: Riaan
Thank you for your question. We do not characterize the isoelectric point for any of our antibodies. The isoelectric point (pI) of IgG is found in the range of 6.4 to 9.0 [1]. References: [1] Li G, Stewart R, Conlan B, Gilbert A, Roerth P, Nair H. Purification of human immunoglobulin G: A new approach to plasma fractionation. Vox Sanguinis (2002) 83, 332-338.
Answered by: Technical Support
Date published: 2019-07-12

For the dye conjugated antibodies (eg. P-selectin-FITC), will further conjugation still be available on that antibody? ie. does the antibody still have primary NH2 groups for other conjugation reactions?

Asked by: mich77
Thank you for your question. Most reactive/readily available primary amines have most likely have been conjugated. We can not say definitely, but there may be some available. However, we would not recommend attempting additional conjugation of the antibody since the antibody conjugates are provided with a stabilizing protein (gelatin) which would interfere with the conjugation.
Answered by: Technical Support
Date published: 2019-06-16

I purchased 4 antibodies against human PECAM, VE-Cadherin, Ang-2 and P-selectin for IF of endothelial cells, all first three antibodies worked well, but the P-selectin ( sc-8419) did not worked. I used same secondary antibodies for all of them.

Asked by: Hamideh
Thank you for your question. We are sorry to hear that you are not getting the expected result with one of our antibodies. Please contact our Technical Service by phone, email or by Live Chat. They will help you figure out how to resolve the issue and provide an appropriate replacement free of charge if the original antibody does not work.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-30

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with P-Selectin Antibody (CTB201): sc-8419?

Asked by: Trav11
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Service 11
Date published: 2017-01-17

Does P-Selectin Antibody (CTB201): sc-8419 react with canine samples?

Asked by: Trav11
Thank you for your question. For additional species reactivity not listed on our website, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or by using the Live Chat function on our website and we will be more than happy to run sequence analysis for your species of interest.
Answered by: Technical Support 5
Date published: 2017-01-16
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Rated 1 out of 5 by from Tested on HFFF by FACS This antibody was tested on HFFF cells by FACS and did not give a signal, even at dilution 1:25
Date published: 2018-11-19
Rated 5 out of 5 by from Excellent Antibodies are used in human platelet extract, which are better than what I ordered from other suppliers and strong signals are diluted at high concentrations
Date published: 2017-10-31
Rated 5 out of 5 by from clear band Obtained a single clear band in Western Blot with 293T cells.
Date published: 2017-02-25
Rated 5 out of 5 by from Superb data Works well for FCM, IF and western blot applications
Date published: 2017-01-24
Rated 5 out of 5 by from Great monoclonal choice for P-Selectin Strong and specific, it's a great choice for IHC(P), FCM, IF or WB applications.
Date published: 2017-01-17
Rated 5 out of 5 by from Works very good excellent antibody, works by western blot at 1:1000 for both mouse and human lysates
Date published: 2016-12-17
Rated 5 out of 5 by from very good We often use P-Selectin antibody,used for Western blot detection of platelet extraction,always use domestic brands antibody, are colleagues recommend Santa monoclonal antibodies,Effect is very good.
Date published: 2016-12-17
Rated 5 out of 5 by from Use this routinely in WB and works well Use this routinely in WB and works well.
Date published: 2015-07-10
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