p-Rsk Antibody (C-5) recommended for detection of Ser 380 phosphorylated Rsk of mouse, rat and human origin by WB, IP, IF and ELISA

p-Rsk Antibody (C-5): sc-377526

p-Rsk Antibody (C-5) is rated 4.5 out of 5 by 4.
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Datasheets

    • p-Rsk Antibody (C-5) is a mouse monoclonal IgG2a κ, cited in 1 publications, provided at 200 µg/ml
    • specific for an epitope mapping to a short amino acid sequence containing phosphorylated Ser 380 of Rsk -1 of human origin
    • Anti-p-Rsk Antibody (C-5) is recommended for detection of Ser 380 phosphorylated Rsk of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-p-Rsk Antibody (C-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p-Rsk (C-5): sc-377526.

What application is the blocking peptide sc-377526 P appropriate for?

Asked by: Professor Griffin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Muy buen resultado para WB Muy buen resultado para WB. La banda es específica en extractos celulares totales de células AGS.
Date published: 2018-01-11
Rated 4 out of 5 by from Works well for WB works well for western - samples were human cells in culture
Date published: 2017-11-21
Rated 4 out of 5 by from Good antibody for Western blotting This monoclonal antibody was independently tested by Kinexus Bioinformatics Corporation and was determined to be suitable for Western blotting. It provided medium detection of the Rsk target in immunoblots of lysates from HeLa cells that were exposed to anisomycin for 2 hours, but no other cross-reactivities were observed. There was no detection of the Rsk target in overnight serum-starved human A431 cells treated for 5 minutes with epidermal growth factor on Western blots, but again this antibody was very clean and no significant cross-reactivities on these blots with any off-target proteins.
Date published: 2016-12-08
Rated 5 out of 5 by from Nice Western blot data of Rsk phosphorylation Nice Western blot data of Rsk phosphorylation in untreated, PMA treated and PMA and lambda protein phosphatase treated HeLa whole cell lysates. -SCBT QC
Date published: 2013-11-03
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