p-Rsk-2 Antibody (C-6): sc-377501

p-Rsk-2 Antibody (C-6) is a mouse monoclonal IgG2b kappa light chain antibody that detects Thr 577 phosphorylated Rsk-2 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). p-Rsk-2 (C-6) antibody is available in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. Rsk-2, a member of the ribosomal S6 kinase family, plays a crucial role in cellular signaling pathways, particularly in response to receptor tyrosine kinase activation. Rsk-2 is primarily located in the cytoplasm, where Rsk-2 acts as a key mediator of mitogen-activated protein kinase (MAPK) signaling, influencing processes such as cell growth, differentiation, and survival. Proper localization of Rsk-2 is essential for function, as localization allows for timely phosphorylation of downstream targets that regulate gene expression and cellular responses to external stimuli. Rsk-2′s unique structure, featuring two non-identical kinase catalytic domains, enables interaction with various substrates and signaling molecules, further underscoring Rsk-2′s importance in cellular signaling networks. p-Rsk-2 (C-6) monoclonal antibody′s specific detection of phosphorylated Rsk-2 makes p-Rsk-2 (C-6) antibody an invaluable tool for researchers studying cell signaling mechanisms and Rsk-2′s role in various physiological and pathological processes.

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p-Rsk-2 Antibody (C-6) References:

1. RSK2 activity is regulated by its interaction with PEA-15.  |  Vaidyanathan, H. and Ramos, JW. 2003. J Biol Chem. 278: 32367-72. PMID: 12796492
2. Functional characterization of human RSK4, a new 90-kDa ribosomal S6 kinase, reveals constitutive activation in most cell types.  |  Dümmler, BA., et al. 2005. J Biol Chem. 280: 13304-14. PMID: 15632195
3. Cloning of the mitogen-activated S6 kinase from rat liver reveals an enzyme of the second messenger subfamily.  |  Kozma, SC., et al. 1990. Proc Natl Acad Sci U S A. 87: 7365-9. PMID: 1699226
4. RSK2 mediates muscle cell differentiation through regulation of NFAT3.  |  Cho, YY., et al. 2007. J Biol Chem. 282: 8380-92. PMID: 17213202
5. Molecular structure of a major insulin/mitogen-activated 70-kDa S6 protein kinase.  |  Banerjee, P., et al. 1990. Proc Natl Acad Sci U S A. 87: 8550-4. PMID: 2236064
6. Identification of mitogen-responsive ribosomal protein S6 kinase pp90rsk, a homolog of Xenopus S6 kinase II, in chicken embryo fibroblasts.  |  Sweet, LJ., et al. 1990. Mol Cell Biol. 10: 2413-7. PMID: 2325657
7. p90 RSK2 mediates antianoikis signals by both transcription-dependent and -independent mechanisms.  |  Jin, L., et al. 2013. Mol Cell Biol. 33: 2574-85. PMID: 23608533
8. Sequence and expression of chicken and mouse rsk: homologs of Xenopus laevis ribosomal S6 kinase.  |  Alcorta, DA., et al. 1989. Mol Cell Biol. 9: 3850-9. PMID: 2779569
9. RSK1 and RSK2 as therapeutic targets: an up-to-date snapshot of emerging data.  |  Spirrison, AN. and Lannigan, DA. 2024. Expert Opin Ther Targets. 28: 1047-1059. PMID: 39632509
10. RSK3 encodes a novel pp90rsk isoform with a unique N-terminal sequence: growth factor-stimulated kinase function and nuclear translocation.  |  Zhao, Y., et al. 1995. Mol Cell Biol. 15: 4353-63. PMID: 7623830
11. Divergent functional roles for p90rsk kinase domains.  |  Bjørbaek, C., et al. 1995. J Biol Chem. 270: 18848-52. PMID: 7642538
12. Human rsk isoforms: cloning and characterization of tissue-specific expression.  |  Moller, DE., et al. 1994. Am J Physiol. 266: C351-9. PMID: 8141249

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-Rsk-2 Antibody (C-6) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-p-Rsk-2 Antibody (C-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-Rsk-2 Antibody (C-6) Specifications

Alternate Product Name: p-RPS6KA3

Product Clone Name: C-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: Thr 577

Epitope Target: Rsk-2

Epitope Species: human

Product Reactivity/Detection: Thr 577 phosphorylated Rsk-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for p-Rsk-2 Antibody (C-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred Rsk-2 antibody is Rsk-2 Antibody (E-1): sc-9986.