p-Rsk-2 Antibody (C-6) recommended for detection of Thr 577 phosphorylated Rsk-2 of mouse, rat, human and avian origin by WB, IP, IF, IHC(P) and ELISA

p-Rsk-2 Antibody (C-6): sc-377501

p-Rsk-2 Antibody (C-6) is rated 4.3 out of 5 by 3.
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Datasheets

    • p-Rsk-2 Antibody (C-6) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • p-Rsk-2 Antibody (C-6) is recommended for detection of Thr 577 phosphorylated Rsk-2 of mouse, rat, human and avian origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
    • Anti-p-Rsk-2 Antibody (C-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-377501 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p-Rsk-2 (C-6): sc-377501.

What application is the blocking peptide sc-377501 P appropriate for?

Asked by: DefinitelyNotMatt
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-03-01
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Rated 4 out of 5 by from 背景较深 I bought the anti0body for HepG2 cells two month ago. It showed good expression but always has unclean background.
Date published: 2017-08-29
Rated 4 out of 5 by from Potent and fairly specific antibody This monoclonal antibody was independently tested by Kinexus Bioinformatics Corporation and is recommended for Western blotting. There was very strong detection of ~80-84 kDa RSK2 in overnight serum-starved human A431 cells treated for 5 minutes with EGF on Western blots, and no significant cross-reactivities on these blots with any off-target proteins, except for some that migrated above 100 kDa.
Date published: 2017-03-14
Rated 5 out of 5 by from Great Western blot data of Rsk Great Western blot data of Rsk-2 phosphorylation in untreated, PMA treated and PMA and lambda protein phosphatase treated HeLa whole cell lysates. -SCBT QC
Date published: 2013-04-07
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