p-RKIP Antibody (80.Ser 153): sc-135779

p-RKIP (80.Ser 153) is a mouse monoclonal IgG2a kappa light chain antibody that detects Ser 153 phosphorylated RKIP of human origin by western blotting (WB) and enzyme-linked immunosorbent assay (ELISA). anti-p-RKIP antibody (80.Ser 153) is available in both conjugated and non-conjugated forms, providing flexibility for various experimental needs. Raf kinase inhibitory protein (RKIP), also known as PEBP, plays a crucial role in modulating the Raf/MAPK signaling pathway, which is vital for regulating cell growth, differentiation, and survival. Phosphorylation of RKIP at Serine 153 influences RKIP′s ability to interact with other proteins in the signaling cascade. When protein kinase C phosphorylates RKIP in response to stimuli such as phorbol esters or epidermal growth factor, RKIP undergoes a conformational change that allows dissociation from Raf-1 and association with G protein-coupled receptor kinase 2 (GRK2). This interaction effectively inhibits GRK2 activity, thereby impacting downstream signaling events. RKIP′s ability to modulate these pathways is essential for tumor suppressor function, inhibiting NFkB activation in response to inflammatory cytokines like TNF-α and interleukin-1 beta, ultimately influencing cancer progression and metastasis.

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p-RKIP Antibody (80.Ser 153) References:

1. Suppression of Raf-1 kinase activity and MAP kinase signalling by RKIP.  |  Yeung, K., et al. 1999. Nature. 401: 173-7. PMID: 10490027
2. Mechanism of suppression of the Raf/MEK/extracellular signal-regulated kinase pathway by the raf kinase inhibitor protein.  |  Yeung, K., et al. 2000. Mol Cell Biol. 20: 3079-85. PMID: 10757792
3. Crystal structures of YBHB and YBCL from Escherichia coli, two bacterial homologues to a Raf kinase inhibitor protein.  |  Serre, L., et al. 2001. J Mol Biol. 310: 617-34. PMID: 11439028
4. Raf kinase inhibitor protein interacts with NF-kappaB-inducing kinase and TAK1 and inhibits NF-kappaB activation.  |  Yeung, KC., et al. 2001. Mol Cell Biol. 21: 7207-17. PMID: 11585904
5. Activation of Raf-1 signaling by protein kinase C through a mechanism involving Raf kinase inhibitory protein.  |  Corbit, KC., et al. 2003. J Biol Chem. 278: 13061-8. PMID: 12551925
6. Protein kinase C switches the Raf kinase inhibitor from Raf-1 to GRK-2.  |  Lorenz, K., et al. 2003. Nature. 426: 574-9. PMID: 14654844
7. Inhibition of the Raf-MEK1/2-ERK1/2 signaling pathway, Bcl-xL down-regulation, and chemosensitization of non-Hodgkin's lymphoma B cells by Rituximab.  |  Jazirehi, AR., et al. 2004. Cancer Res. 64: 7117-26. PMID: 15466208
8. The biology of a prostate cancer metastasis suppressor protein: Raf kinase inhibitor protein.  |  Keller, ET., et al. 2005. J Cell Biochem. 94: 273-8. PMID: 15565643
9. Raf kinase inhibitory protein regulates Raf-1 but not B-Raf kinase activation.  |  Trakul, N., et al. 2005. J Biol Chem. 280: 24931-40. PMID: 15886202
10. Expression of phosphorylated raf kinase inhibitor protein (pRKIP) is a predictor of lung cancer survival.  |  Huerta-Yepez, S., et al. 2011. BMC Cancer. 11: 259. PMID: 21689459
11. CDK5-mediated phosphorylation and autophagy of RKIP regulate neuronal death in Parkinson's disease.  |  Wen, Z., et al. 2014. Neurobiol Aging. 35: 2870-2880. PMID: 25104559
12. Protein kinase C-mediated phosphorylation of RKIP regulates inhibition of Na-alanine cotransport by leukotriene D(4) in intestinal epithelial cells.  |  Arthur, S. and Sundaram, U. 2014. Am J Physiol Cell Physiol. 307: C1010-6. PMID: 25231108

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-RKIP Antibody (80.Ser 153) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-p-RKIP Antibody (80.Ser 153) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-RKIP Antibody (80.Ser 153) Specifications

Alternate Product Name: p-PEBP1

Product Clone Name: 80.Ser 153

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: Ser 153

Epitope Target: RKIP

Epitope Species: human

Product Reactivity/Detection: Ser 153 phosphorylated RKIP

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for p-RKIP Antibody (80.Ser 153): AC: Agarose, HRP: Horseradish Peroxidase

The preferred RKIP antibody is RKIP Antibody (H-10): sc-376925.