p-RKIP Antibody (80.Ser 153) recommended for detection of Ser 153 phosphorylated RKIP of human origin by WB and ELISA

p-RKIP Antibody (80.Ser 153): sc-135779

p-RKIP Antibody (80.Ser 153) is rated 4.0 out of 5 by 4.
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Datasheets

    • p-RKIP Antibody (80.Ser 153) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against a short amino acid sequence containing phosphorylated Ser 153 of RKIP of human origin
    • recommended for detection of Ser 153 phosphorylated RKIP of human origin by WB and ELISA
    • available conjugated to agarose for IP; and to HRP for WB, IHC(P) and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p-RKIP (80.Ser 153): sc-135779.

Gent.mi,  How did you check the specificity of this antibody?  We are testing it by ELISA but It seems that It recognizes both native and p-153 RKIP with the same affinity...It should not happen. 

Asked by: Marrabio10
Thank you for your question. p-RKIP Antibody (80.Ser 153): sc-135779 has been validated by WB and ELISA for specificity against Ser 153 phosphorylated RKIP of human origin. Please contact our Technical Service Department for help with troubleshooting the antibody if you have results that indicate the antibody is non-specifically recognizing the non-phosphorylated antigen. Please call our European Support office toll-free at 00800 4573 8000, email [email protected], or contact us by live chat.
Answered by: Technical Support
Date published: 2017-05-24

How much sample do you recommend loading on the gel for Western blot detection using p-RKIP (80.Ser 153): sc-135779?

Asked by: jenni
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-27
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Rated 4 out of 5 by from Good antibody but have a week band at liver sample Good antibody but have a week band at normal liver sample
Date published: 2017-12-04
Rated 5 out of 5 by from Works well for Western blotting Have used this antibody on Western blots and it works very well. Would recommend for Westerns.
Date published: 2017-07-21
Rated 5 out of 5 by from Potent and very specific antibody This monoclonal antibody was independently tested by Kinexus Bioinformatics Corporation and is recommended for Western blotting. There was very strong detection of RKIP in 30 minute anisomycin-treated human HeLa cells on Western blots, and no significant cross-reactivities on these blots with any off-target proteins.
Date published: 2017-03-14
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