Anti-p-Rb Antibody (A-5) recommended for detection of Ser 249 and Thr 252 dually-phosphorylated Rb of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-p-Rb Antibody (A-5): sc-377528

Anti-p-Rb Antibody (A-5) is rated 3.9 out of 5 by 7.
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Datasheets

    • p-Rb Antibody (A-5) is a mouse monoclonal IgG2b κ, cited in 14 publications, provided at 200 µg/ml
    • Anti-p-Rb Antibody (A-5) is recommended for detection of Ser 249 and Thr 252 dually-phosphorylated Rb of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-p-Rb Antibody (A-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p-Rb (A-5): sc-377528.
    • m-IgG Fc BP-HRP and m-IgG2b BP-HRP are the preferred secondary detection reagents for p-Rb Antibody (A-5) for WB and IHC(P) applications. These reagents are now offered in bundles with p-Rb Antibody (A-5) (see ordering information below).
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Could you please provide the concentration of the glue of WB, the membrane transfer conditions, the type of sealing liquid and the sealing time, and the primary concentration?

Asked by: lily 12
Thank you for your question. For the transfer conditions, please reach out to the manufacturer of your transfer equipment. The recommended starting dilution of this primary antibody is 1:50 dilution. Here is a link to our recommended protocol. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Tech Service
Date published: 2019-09-06

What application is the blocking peptide sc-377528 P appropriate for?

Asked by: Randy McDonald
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from Didnot work for WB did not work for western - samples were human cells in culture
Date published: 2017-11-21
Rated 3 out of 5 by from not good for fish protein some unspecific bands showed when do the fish experiment.
Date published: 2017-08-08
Rated 5 out of 5 by from perfect antibody. I applied sc-377528 sample half a month ago to test the changes of p-Rb in my cell after treatment and happy to find a good result.
Date published: 2017-08-08
Rated 4 out of 5 by from very good there was three bands in turbot, primary antibody 1:200, second antibody 1:10000. good enough
Date published: 2017-06-20
Rated 4 out of 5 by from Fairly potent and specific antibody This monoclonal antibody was independently tested by Kinexus Bioinformatics Corporation and is recommended for Western blotting. There was medium detection of a ~105 kDa target-sized protein in overnight serum-starved human A431 cells treated for 5 minutes with EGF on Western blots, and only significant cross-reactivity with ~45 kDa protein.
Date published: 2016-12-04
Rated 5 out of 5 by from Good bands in Western Blot acquired Good bands in Western Blot acquired. -SCBT QC
Date published: 2015-05-01
Rated 5 out of 5 by from Beautiful immunoperoxidase nuclear and cytoplasmic Beautiful immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human vagina tissue. -SCBT QC
Date published: 2015-03-22
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