p-NF-H Antibody (RNF404): sc-32728

p-NF-H Antibody (RNF404) is a mouse monoclonal IgG2a antibody that detects neurofilament-H in mouse, rat, human, Xenopus, and bovine samples through western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF) applications. Anti-neurofilament-H antibody (RNF404) specifically recognizes phosphorylated forms of neurofilament-H, which is a crucial component of the neuronal cytoskeleton, playing a significant role in maintaining the structural integrity and function of neurons. Neurofilament-H (RNF404) monoclonal antibody is particularly important for studying axon caliber regulation, which directly influences nerve impulse conduction speed. Phosphorylation of neurofilament-H affects interactions with other cytoskeletal elements, such as microtubules and actin filaments, thereby impacting overall neuronal stability and signaling. Alterations in neurofilament-H phosphorylation are often associated with various neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS), Parkinson′s disease, and Alzheimer′s disease, making neurofilament-H research vital for understanding these conditions. With broad species reactivity and proven applications, anti-neurofilament-H antibody (RNF404) serves as an essential tool for investigating neuronal health and disease.

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p-NF-H Antibody (RNF404) References:

1. Elevated levels of phosphorylated neurofilament proteins in cerebrospinal fluid of Alzheimer disease patients.  |  Hu, YY., et al. 2002. Neurosci Lett. 320: 156-60. PMID: 11852185
2. Effect of the branched-chain alpha-ketoacids accumulating in maple syrup urine disease on the high molecular weight neurofilament subunit (NF-H) in rat cerebral cortex.  |  Pessoa-Pureur, R., et al. 2002. Metab Brain Dis. 17: 65-75. PMID: 12083338
3. Effect of propionic and methylmalonic acids on the high molecular weight neurofilament subunit (NF-H) in rat cerebral cortex.  |  Vivian, L., et al. 2002. Neurochem Res. 27: 1691-7. PMID: 12515324
4. Deleting the phosphorylated tail domain of the neurofilament heavy subunit does not alter neurofilament transport rate in vivo.  |  Yuan, A., et al. 2006. Neurosci Lett. 393: 264-8. PMID: 16266786
5. Expression of the neurofilament protein NF-H in L cells.  |  Soifer, D., et al. 1991. J Neurosci Res. 30: 63-71. PMID: 1795408
6. Neurofilament protein levels: quantitative analysis in essential tremor cerebellar cortex.  |  Louis, ED., et al. 2012. Neurosci Lett. 518: 49-54. PMID: 22561033
7. Early expression of the high molecular weight neurofilament subunit attenuates axonal neurite outgrowth.  |  Boumil, E., et al. 2015. Neurosci Lett. 604: 36-41. PMID: 26225928
8. A Pilot Cross-Sectional Study to Investigate the Biomarker Potential of Phosphorylated Neurofilament-H and Immune Mediators of Disability in Patients With 5 Year Relapsing-Remitting Multiple Sclerosis.  |  Herrera, MI., et al. 2019. Front Neurol. 10: 1046. PMID: 31649604
9. Microfluidic Platforms Promote Polarization of Human-Derived Retinal Ganglion Cells That Model Axonopathy.  |  Boal, AM., et al. 2023. Transl Vis Sci Technol. 12: 1. PMID: 37010860
10. Phosphorylation and dephosphorylation of distinct isoforms of the heavy neurofilament protein NF-H.  |  Chertoff, R., et al. 1995. Cell Mol Neurobiol. 15: 269-81. PMID: 8590456

The preferred NF-H antibody is NF-H Antibody (RNF402): sc-32729.