NF-H Antibody (RNF402): sc-32729

NF-H Antibody (RNF402) is a mouse monoclonal IgM antibody that detects NF-H protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-NF-H antibody (RNF402) is available as the non-conjugated format. NF-H protein, or neurofilament heavy polypeptide, is a crucial component of the neuronal cytoskeleton, playing a significant role in maintaining neuronal structural integrity. Located primarily in axons of neurons, NF-H contributes to axon diameter regulation, which is essential for proper nerve impulse conduction. Dynamic neurofilaments interact with other cytoskeletal components, such as microtubules and actin filaments, influencing overall neuronal function and stability. Alterations in NF-H expression or phosphorylation are often associated with various neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS), Parkinson′s disease, and Alzheimer′s disease, making NF-H a valuable biomarker for studying these conditions.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

NF-H Antibody (RNF402) References:

1. Casein kinase II is responsible for phosphorylation of NF-L at Ser-473.  |  Nakamura, Y., et al. 1999. FEBS Lett. 455: 83-6. PMID: 10428477
2. Neurofilament metabolism in sporadic amyotrophic lateral sclerosis.  |  Strong, MJ. 1999. J Neurol Sci. 169: 170-7. PMID: 10540027
3. Protein-chemical characterization of NF-H, the largest mammalian neurofilament component; intermediate filament-type sequences followed by a unique carboxy-terminal extension.  |  Geisler, N., et al. 1985. EMBO J. 4: 57-63. PMID: 16453600
4. Effects of phosphorylation of the neurofilament L protein on filamentous structures.  |  Hisanaga, S., et al. 1990. Cell Regul. 1: 237-48. PMID: 2100199
5. Involvement of protein kinase C in the regulation of assembly-disassembly of neurofilaments in vitro.  |  Gonda, Y., et al. 1990. Biochem Biophys Res Commun. 167: 1316-25. PMID: 2108674
6. In vivo phosphorylation of distinct domains of the 70-kilodalton neurofilament subunit involves different protein kinases.  |  Sihag, RK. and Nixon, RA. 1989. J Biol Chem. 264: 457-64. PMID: 2491851
7. Influence of a GSK3β phosphorylation site within the proximal C-terminus of Neurofilament-H on neurofilament dynamics.  |  Vohnoutka, RB., et al. 2017. Biol Open. 6: 1516-1527. PMID: 28882840
8. Assembly and exchange of intermediate filament proteins of neurons: neurofilaments are dynamic structures.  |  Angelides, KJ., et al. 1989. J Cell Biol. 108: 1495-506. PMID: 2925792
9. Monoclonal antibodies distinguish several differentially phosphorylated states of the two largest rat neurofilament subunits (NF-H and NF-M) and demonstrate their existence in the normal nervous system of adult rats.  |  Lee, VM., et al. 1987. J Neurosci. 7: 3474-88. PMID: 3119789
10. The gene encoding the large human neurofilament subunit (NF-H) maps to the q121-q131 region on human chromosome 22.  |  Mattei, MG., et al. 1988. Hum Genet. 80: 293-5. PMID: 3192217
11. Changes in neurofilament protein NF-L and NF-H immunoreactivity following kainic acid-induced seizures.  |  Wang, S., et al. 1994. J Neurochem. 62: 739-48. PMID: 8294936
12. Abnormal distribution of neurofilament L in neurons with Alzheimer's disease.  |  Nakamura, Y., et al. 1997. Neurosci Lett. 225: 201-4. PMID: 9147405

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NF-H Antibody (RNF402) has 19 citations in a variety of scientific publications.

Primary antibodies like Anti-NF-H Antibody (RNF402) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NF-H Antibody (RNF402) Specifications

Alternate Product Name: NEFH

Product Clone Name: RNF402

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids:

Epitope Target: NF-H

Epitope Species: bovine

Product Reactivity/Detection: NF-H

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: bovine

Additional Non-Mammalian Reactivity: Xenopus

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 19 citations in various scientific publications

Available Conjugates/Forms for NF-H Antibody (RNF402): N/A