p-INSR/Insulin Receptor β Antibody (10C3): sc-81500

p-insulin Rβ Antibody (10C3) is a mouse monoclonal IgG1 kappa light chain antibody that detects p-insulin Rβ in mouse, rat, and human samples through western blotting (WB) and immunoprecipitation (IP) applications. p-insulin Rβ (10C3) antibody is available in a non-conjugated form, specifically designed for detecting dually phosphorylated Tyr 1150 and Tyr 1151 of the insulin receptor β subunit and IGF1 receptor. The insulin receptor (IR) plays a crucial role in cellular metabolism by mediating insulin effects, a key hormone in glucose homeostasis. Located on the cell membrane, IR is a heterodimeric protein complex composed of an extracellular α subunit and an intracellular β subunit, which is essential for receptor function. Upon insulin binding, IR undergoes a conformational change that activates intrinsic tyrosine kinase activity, leading to phosphorylation of specific tyrosine residues within the β subunit. This phosphorylation event facilitates recruitment of various signaling proteins that propagate the insulin signal, ultimately promoting glucose uptake and glycogen synthesis in target tissues. The human insulin receptor gene encodes a 1,382 amino acid protein that is cleaved to form functional α and β subunits, and undergoes post-translational modifications, such as phosphorylation at Tyr 1322, which further modulates activity and signaling capabilities.

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p-INSR/Insulin Receptor β Antibody (10C3) References:

1. Signalling through the insulin receptor.  |  Whitehead, JP., et al. 2000. Curr Opin Cell Biol. 12: 222-8. PMID: 10712920
2. The insulin receptor: from protein sequence to structure.  |  Marino-Buslje, C., et al. 1999. Biochem Soc Trans. 27: 715-26. PMID: 10917671
3. Mechanism of transmembrane signaling: insulin binding and the insulin receptor.  |  Ottensmeyer, FP., et al. 2000. Biochemistry. 39: 12103-12. PMID: 11015187
4. Insulin receptor--structural and functional characteristics.  |  Perz, M. and Torlińska, T. 2001. Med Sci Monit. 7: 169-77. PMID: 11208515
5. Insulin receptor variant forms and type 2 diabetes mellitus.  |  Sesti, G. 2000. Pharmacogenomics. 1: 49-61. PMID: 11258596
6. Phosphorylated insulin-like growth factor-i/insulin receptor is present in all breast cancer subtypes and is related to poor survival.  |  Law, JH., et al. 2008. Cancer Res. 68: 10238-46. PMID: 19074892
7. Co-expression of matrix metalloproteinase-7 (MMP-7) and phosphorylated insulin growth factor receptor I (pIGF-1R) correlates with poor prognosis in patients with wild-type KRAS treated with cetuximab or panitumumab: a GEMCAD study.  |  Hörndler, C., et al. 2011. Cancer Biol Ther. 11: 177-83. PMID: 21099348
8. Cervical phosphorylated insulin-like growth factor binding protein-1 test for the prediction of preterm birth: a systematic review and metaanalysis.  |  Conde-Agudelo, A. and Romero, R. 2016. Am J Obstet Gynecol. 214: 57-73. PMID: 26149828
9. Phosphorylated-insulin growth factor I receptor (p-IGF1R) and metalloproteinase-3 (MMP3) expression in advanced gastrointestinal stromal tumors (GIST). A GEIS 19 study.  |  Maurel, J., et al. 2016. Clin Sarcoma Res. 6: 10. PMID: 27358721
10. ZFYVE28 mediates insulin resistance by promoting phosphorylated insulin receptor degradation via increasing late endosomes production.  |  Yu, L., et al. 2023. Nat Commun. 14: 6833. PMID: 37884540

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-INSR/Insulin Receptor β Antibody (10C3) has 41 citations in a variety of scientific publications.

Primary antibodies like Anti-p-INSR/Insulin Receptor β Antibody (10C3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-INSR/Insulin Receptor β Antibody (10C3) Specifications

Alternate Product Name: Insulin Receptor Beta

Product Clone Name: 10C3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: Tyr 1150/1151

Epitope Target: insulin R

Epitope Species: human

Product Reactivity/Detection: Tyr 1150 and Tyr 1151 dually phosphorylated insulin Rβ and IGF1 receptor

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 50 µg IgG₁ kappa light chain in 0.5 ml of PBS with < 0.1% sodium azide, 0.1% gelatin, PEG and sucrose

Research Citations: 41 citations in various scientific publications

Available Conjugates/Forms for p-INSR/Insulin Receptor β Antibody (10C3): N/A

The preferred INSR/Insulin Receptor β antibody is INSR/Insulin Receptor β Antibody (CT-3): sc-57342.