insulin R beta Antibody (CT-3) recommended for detection of insulin Rβ of mouse, rat and human origin by WB, IP, IF and IHC(P)

insulin Rβ Antibody (CT-3): sc-57342

insulin Rβ Antibody (CT-3) is rated 4.3 out of 5 by 18.
  • y_2020, m_4, d_2, h_16
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.5
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_18
  • loc_en_US, sid_57342, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 148ms
  • REVIEWS, PRODUCT
5
10
4
6
3
1
2
0
1
1

Datasheets

    • Anti-insulin Rβ Antibody (CT-3) is a mouse monoclonal IgG1 (kappa light chain) insulin Rβ antibody provided at 200 µg/ml
    • raised against the C-terminus of insulin receptor of human origin
    • Anti-insulin R beta Antibody (CT-3) is recommended for detection of insulin Rβ of mouse, rat and human origin by WB, IP, IF and IHC(P); non cross-reactive with insulin-like growth factor (IGF)receptors
    • Anti-insulin R beta Antibody (CT-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of insulin Rβ (CT-3): sc-57342.
Submit a review for this product and receive 15 CruzCredits

Is a blocking pepide available for the sc-57342 Insulin receptor antibody?

Asked by: PaulDA
Thank you for your question. There is no blocking peptide available for this specific antibody.
Answered by: Technical Support
Date published: 2019-09-11

Can you provide information about the hybridoma cell line this antibody was produced from?

Asked by: KMitok
Thank you for your question. The specific clone name for this monoclonal antibody is CT-3. If you require additional information, please contact our Technical Service Department for assistance.
Answered by: Technical Support
Date published: 2019-09-11

I have a clear band of approximately 45 KDa with this antibody. Do you know what it can be? Thank you so much!

Asked by: Jimena
Thank you for your question. We do not have any information regarding a 45 kDa sized band when using this antibody. Please contact our Technical Support Department for help with troubleshooting the antibody, or more detailed information about the expected molecular weight. Please call us toll-free at (800) 457-3801 option 2, or use the live chat on our website.
Answered by: Technical Support
Date published: 2018-11-22

In the data sheet , it was written the antigen is C-terminal , does the C-terminal region contain Tryosine domain?

Asked by: TANG QIAN
Thank you for your question. This antibody was made against a recombinant protein fragment including the C-terminal 100 amino acid residues of human insulin receptor (accession#P06213). There are tyrosine domains within the C-terminus but since the antibody has not been epitope mapped, I do not know if the exact epitope includes a Tyrosine. If you have any further questions, please contact our Asia Technical Service team. You can reach them by phone at (86 21) 6093-6350, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-09-05

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with insulin Rβ (CT-3): sc-57342 monoclonal antibody?

Asked by: jenni
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 4
Date published: 2017-01-10
  • y_2020, m_4, d_2, h_16CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.5
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_5
  • loc_en_US, sid_57342, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 149ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from Application of insulin Rβ Antibody for IHC and WB The insulin Rβ Antibody (CT-3) SC-57342 is suitable for intracellular localization studies of the insulin receptor in 3T3-L1 adipocytes at different stages of differentiation by immunofluorescence and confocal microscopy . The antibody can be also used for Western blot studies of protein extracts from 3T3-L1 adipocytes, including quantitative analyses.
Date published: 2018-09-13
Rated 5 out of 5 by from Works great! Works at 1:50 dilution for immunofluorescence in hES cells
Date published: 2018-03-01
Rated 5 out of 5 by from Works great for IHC in frozen mouse liver sections I used this at a 1:250 dilution on frozen mouse liver sections and it worked great! Did not work well in paraffin embedded sections.
Date published: 2018-01-03
Rated 4 out of 5 by from nice band the antibody was tested in western blot on rat tissue samples and worked well
Date published: 2017-12-13
Rated 4 out of 5 by from positive result in WB worked well for detection of insulin receptor from cell lysate
Date published: 2017-09-06
Rated 3 out of 5 by from ISR b This antibody was used for Western blotting of MCF7 and T47D cell lysate。It did not work in T47D。It had some signal with a little background in MCF7.
Date published: 2017-06-15
Rated 4 out of 5 by from relative high sensitivity show some cross reaction with IGF-1Rb, but generally ok to detect IRb
Date published: 2017-05-17
Rated 1 out of 5 by from Confronto tra anticorpo poli e monoclonale A parità di proteine caricate (40ug) e condizioni di diluizione anticorpo (1:1000 in milk 2.5%) il nuovo anticorpo monoclonale (sc-57342) non funziona in modo soddisfacente rispetto al vecchio anticorpo policlonale (sc-711).
Date published: 2017-04-03
  • y_2020, m_4, d_2, h_16
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.5
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_18
  • loc_en_US, sid_57342, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 9ms
  • REVIEWS, PRODUCT

Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2020 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.