p-GSK3 alpha/beta Antibody (6D3): sc-81496

p-GSK-3α/β Antibody (6D3) is a mouse monoclonal IgG1 kappa light chain antibody that detects p-GSK-3α/β in mouse, rat, and human samples through western blotting (WB) and immunoprecipitation (IP) applications. anti-p-GSK-3α/β antibody (6D3) is available in a non-conjugated form, specifically designed for researchers studying the phosphorylation states of glycogen synthase kinase-3α (GSK-3α) and GSK-3β, which are critical serine/threonine kinases involved in various cellular processes. GSK-3α and GSK-3β play essential roles in regulating metabolic enzymes and transcription factors, thereby influencing glycogen synthesis and cell adhesion. GSK-3β is crucial for the nuclear activity of Rel dimers, which mediate an anti-apoptotic response to tumor necrosis factor alpha (TNFα) in mice, highlighting GSK-3β′s importance in cell survival pathways. GSK-3 activity is regulated through phosphorylation at specific serine, threonine, and tyrosine residues, with tyrosine phosphorylation at Tyr 279 for GSK-3α and Tyr 216 for GSK-3β serving as activating modifications. GSK-3 interacts with cAMP-dependent protein kinase A (PKA), which phosphorylates GSK-3α and GSK-3β, leading to their inactivation. Dynamic regulation of GSK-3 through these phosphorylation events is vital for maintaining cellular homeostasis and responding to growth factor signals, making p-GSK-3α/β (6D3) monoclonal antibody an invaluable tool for researchers investigating the intricate signaling pathways that govern cell metabolism, development, and survival.

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p-GSK3 alpha/beta Antibody (6D3) References:

1. Glycogen synthase kinase-3beta phosphorylation of MAP1B at Ser1260 and Thr1265 is spatially restricted to growing axons.  |  Trivedi, N., et al. 2005. J Cell Sci. 118: 993-1005. PMID: 15731007
2. Glycogen synthase kinase-3beta inhibits the xenobiotic and antioxidant cell response by direct phosphorylation and nuclear exclusion of the transcription factor Nrf2.  |  Salazar, M., et al. 2006. J Biol Chem. 281: 14841-51. PMID: 16551619
3. Atrogin-1, MuRF1, and FoXO, as well as phosphorylated GSK-3beta and 4E-BP1 are reduced in skeletal muscle of chronic spinal cord-injured patients.  |  Léger, B., et al. 2009. Muscle Nerve. 40: 69-78. PMID: 19533653
4. Age-related changes of Nrf2 and phosphorylated GSK-3β in a mouse model of accelerated aging (SAMP8).  |  Tomobe, K., et al. 2012. Arch Gerontol Geriatr. 54: e1-7. PMID: 21784539
5. Stilbenoid pterostilbene complexed with cyclodextrin preserves left ventricular function after myocardial infarction in rats: possible involvement of thiol proteins and modulation of phosphorylated GSK-3β.  |  Lacerda, D., et al. 2018. Free Radic Res. 52: 988-999. PMID: 30203709
6. Curcumin ameliorates scopolamine-induced mice memory retrieval deficit and restores hippocampal p-Akt and p-GSK-3β.  |  SoukhakLari, R., et al. 2018. Eur J Pharmacol. 841: 28-32. PMID: 30321530
7. A phospholipase D2 inhibitor, CAY10594, ameliorates acetaminophen-induced acute liver injury by regulating the phosphorylated-GSK-3β/JNK axis.  |  Lee, SK., et al. 2019. Sci Rep. 9: 7242. PMID: 31076618
8. Inflammation has synergistic effect with nicotine in periodontitis by up-regulating the expression of α7 nAChR via phosphorylated GSK-3β.  |  Zhou, Z., et al. 2020. J Cell Mol Med. 24: 2663-2676. PMID: 31930698
9. Increasing Odontoblast-like Differentiation from Dental Pulp Stem Cells through Increase of β-Catenin/p-GSK-3β Expression by Low-Frequency Electromagnetic Field.  |  Lim, HM., et al. 2021. Biomedicines. 9: PMID: 34440255
10. Low-Dose Albendazole Inhibits Epithelial-Mesenchymal Transition of Melanoma Cells by Enhancing Phosphorylated GSK-3β/Tyr216 Accumulation.  |  He, Z., et al. 2021. J Oncol. 2021: 4475192. PMID: 34966427

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-GSK3 alpha/beta Antibody (6D3) has 27 citations in a variety of scientific publications.

Primary antibodies like Anti-p-GSK3 alpha/beta Antibody (6D3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-GSK3 alpha/beta Antibody (6D3) Specifications

Alternate Product Name: GSK3

Product Clone Name: 6D3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: Tyr 279

Epitope Target: GSK-3α and tyrosine 216

Epitope Species: human

Product Reactivity/Detection: Tyr 279 phosphorylated GSK-3α and Tyr 216 phosphorylated GSK-3β

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 50 µg IgG₁ kappa light chain in 0.5 ml of PBS with < 0.1% sodium azide, 0.1% gelatin, PEG and sucrose

Research Citations: 27 citations in various scientific publications

Available Conjugates/Forms for p-GSK3 alpha/beta Antibody (6D3): N/A

The preferred GSK3 alpha/beta antibody is GSK3 alpha/beta Antibody (0011-A): sc-7291.