Anti-GSK-3 alpha / beta Antibody (0011-A) recommended for detection of GSK-3α and GSK-3β of mouse, rat, human, canine and Xenopus origin by WB, IP, IF, IHC(P) and FCM

Anti-GSK-3α/β Antibody (0011-A): sc-7291

Anti-GSK-3α/β Antibody (0011-A) is rated 4.9 out of 5 by 46.
  • y_2021, m_6, d_23, h_18
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.17
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_46
  • loc_en_US, sid_7291, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 140ms
  • REVIEWS, PRODUCT
loading
5
40
4
5
3
0
2
0
1
0

Datasheets

    • Anti-GSK-3α/β Antibody (0011-A) is a mouse monoclonal IgG2a κ GSK-3α/β antibody, cited in 230 publications, provided at 200 µg/ml
    • raised against amino acids 1-420 representing full length glycogen synthase kinase-3 beta of Xenopus laevis origin
    • Anti-GSK-3 alpha / beta Antibody (0011-A) is recommended for detection of GSK-3α and GSK-3β of mouse, rat, human, canine and Xenopus origin by WB, IP, IF, IHC(P) and FCM
    • Anti-GSK-3 alpha / beta Antibody (0011-A) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of GSK-3α/β (0011-A): sc-7291.
    • m-IgGκ BP-HRP is the preferred secondary detection reagent for GSK-3α/β Antibody (0011-A) for WB and IHC(P) applications. This reagent is now offered in a bundle with GSK-3α/β Antibody (0011-A) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
loading

How should the cells be fixed for flow cytometry?

Asked by: tubensalami
Thank you for your question. We recommend using a fixation buffer to fix cells for Flow cytometry. Our buffer can be found here: FCM Fixation Buffer (sc-3622) : https://www.scbt.com/scbt/product/fcm-fixation-buffer Our recommended protocol is here: https://www.scbt.com/scbt/resources/protocols/flow-cytometry If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-10-10

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: Cweed
You definitely can. We recommend using a directly conjugated primary antibody. SC-7291 is available directly conjugated to AF488, AF647, and FITC.
Answered by: TechService7
Date published: 2016-12-21

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: Cweed
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: TechService7
Date published: 2016-12-21

Anti-GSK-3α/β Antibody (0011-A): sc-7291. Is this antibody yeast reactive?

Asked by: MichaelS
  • y_2021, m_6, d_23, h_18CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.17
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_4
  • loc_en_US, sid_7291, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 111ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Good antibody for WB I used this antibody diluted 1:500 in milk/TBST buffer, loading 30ug of total protein. The signal is strong, clean bands without any background.
Date published: 2021-06-22
Rated 5 out of 5 by from A clean and potent antibody We purchased the antibody last month for quantification of GSK by Western blotting. We are able to detect both GSK isoforms from HepG2 cells using 2 mcg antibody per blot. Non-specific band was not detected. This is an excellent antibody.
Date published: 2019-10-24
Rated 5 out of 5 by from good product It works pretty well for our western blot experiment.
Date published: 2018-08-22
Rated 4 out of 5 by from Great antibodies I got a free sample from company. I used antibodies in WB in dilution 1:1000. It works good.
Date published: 2018-07-14
Rated 5 out of 5 by from Great antibody for western blot The batch of antibody I have worked well on BJAB, HeLa, MCF7, NIH/3T3, Jurkat and K562 total cell extracts for western Blot. The band is specific. The antibody was diluted 250 times into 5% BSA, TBST and incubated over-night at 4°C.
Date published: 2018-01-04
Rated 5 out of 5 by from Super anticorps !! J'ai tester cet échantillon en WB 1/500 et il marche super bien, signal très spécifique, sans bruit de fond (membrane strippé et réutilisé déjà 3 fois!!).
Date published: 2017-11-30
Rated 5 out of 5 by from Very good - Western Very good signal, clear bands, practically no background (Western blot, melanoma cells)
Date published: 2017-11-03
Rated 5 out of 5 by from Results were great. This is a good WB in MDCK (E) whole cell lysate, I diluted 1:500 with TBST, the results were stable and clear.
Date published: 2017-10-27
  • y_2021, m_6, d_23, h_18
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.17
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_46
  • loc_en_US, sid_7291, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 17ms
  • REVIEWS, PRODUCT