Orexin-A Antibody (KK09): sc-80263

Orexin-A Antibody (KK09) is a mouse monoclonal IgG1 antibody designed to detect Orexin-A across mouse, rat, and human species using western blotting (WB), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). Anti-Orexin-A antibody (KK09) targets a synthetic peptide corresponding to amino acids 34-66 within an internal region of human Orexin-A, ensuring selective binding without cross-reactivity to Orexin-B. Orexin-A, also referred to as Hypocretin-1, HCRT, and PPOX, is a critical hypothalamic neuropeptide derived from the precursor prepro-orexin, playing an essential role in maintaining homeostasis by regulating temperature, thirst, and hunger. Orexin-A exerts effects by binding to Orexin receptor-1 and Orexin receptor-2, which are G protein-coupled receptors involved in the integration of the endocrine and nervous systems. Orexin-A is predominantly localized to neurons in the lateral hypothalamus, known as the "feeding center," and expression is upregulated during fasting, highlighting importance in the central feedback mechanisms that govern feeding behavior. Additionally, Orexin-A enhances the release of neurotransmitters such as GABA and glutamate, facilitating synaptic activities within the hypothalamic region. Orexin-A monoclonal antibody (KK09) is an invaluable tool for researchers studying the physiological and pathological roles of orexins in energy balance, sleep regulation, and neurodegenerative diseases.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Orexin-A Antibody (KK09) References:

1. Role of Orexin-A in Hypertension and Obesity.  |  Imperatore, R., et al. 2017. Curr Hypertens Rep. 19: 34. PMID: 28353077
2. Bidirectional gut-brain communication: A role for orexin-A.  |  Mediavilla, C. 2020. Neurochem Int. 141: 104882. PMID: 33068686
3. Orexin-A measurement in narcolepsy: A stability study and a comparison of LC-MS/MS and immunoassays.  |  Lindström, M., et al. 2021. Clin Biochem. 90: 34-39. PMID: 33539807
4. Soporific effect of modified Suanzaoren Decoction on mice models of insomnia by regulating Orexin-A and HPA axis homeostasis.  |  Dong, YJ., et al. 2021. Biomed Pharmacother. 143: 112141. PMID: 34509822
5. Orexin-A in Patients With Lewy Body Disease: A Systematic Review and Meta-Analysis.  |  Gan, J., et al. 2021. Front Endocrinol (Lausanne). 12: 765701. PMID: 34867809
6. Relation between orexin A and epileptic seizures.  |  Arslan, GA., et al. 2022. Epilepsy Res. 184: 106972. PMID: 35772324
7. Orexin A alleviates LPS-induced acute lung injury by inhibiting macrophage activation through JNK-mediated autophagy.  |  Nie, Y., et al. 2023. Int Immunopharmacol. 124: 111018. PMID: 37801969
8. Therapeutic effects of orexin-A in sepsis-associated encephalopathy in mice.  |  Guo, J., et al. 2024. J Neuroinflammation. 21: 131. PMID: 38760784
9. The hypocretins: hypothalamus-specific peptides with neuroexcitatory activity.  |  de Lecea, L., et al. 1998. Proc Natl Acad Sci U S A. 95: 322-7. PMID: 9419374
10. Orexins and orexin receptors: a family of hypothalamic neuropeptides and G protein-coupled receptors that regulate feeding behavior.  |  Sakurai, T., et al. 1998. Cell. 92: 573-85. PMID: 9491897
11. Orexins: a newly discovered family of hypothalamic regulators of food intake.  |  Wolf, G. 1998. Nutr Rev. 56: 172-3. PMID: 9656726
12. Presynaptic and postsynaptic actions and modulation of neuroendocrine neurons by a new hypothalamic peptide, hypocretin/orexin.  |  van den Pol, AN., et al. 1998. J Neurosci. 18: 7962-71. PMID: 9742163

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Orexin-A Antibody (KK09) has 22 citations in a variety of scientific publications.

Primary antibodies like Anti-Orexin-A Antibody (KK09) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Orexin-A Antibody (KK09) Specifications

Alternate Product Name: Orexin-A

Product Clone Name: KK09

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 34-66

Epitope Target: Orexin-A

Epitope Species: human

Product Reactivity/Detection: Orexin-A

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 50 µg IgG₁ kappa light chain in 0.5 ml of PBS with < 0.1% sodium azide and protein stabilizer

Research Citations: 22 citations in various scientific publications

Available Conjugates/Forms for Orexin-A Antibody (KK09): N/A