OPA1 Antibody (D-9): sc-393296

OPA1 Antibody (D-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects OPA1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). OPA1 Antibody (D-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. OPA1, encoded by the optic atrophy 1 gene located on chromosome 3q29, plays a crucial role in mitochondrial dynamics, particularly in regulating mitochondrial fusion and maintaining mitochondrial morphology. This function is vital for cellular energy production and overall cell health, helping ensure mitochondria can efficiently adapt to changes in energy demand and stress conditions. Defects in OPA1 are associated with optic atrophy type 1, leading to vision loss, and OPA1 is predominantly expressed in the retina, although also found in the brain, testis, heart, and skeletal muscles. OPA1′s ability to interact with other proteins involved in mitochondrial function further underscores OPA1′s importance in cellular metabolism and the pathophysiology of mitochondrial diseases.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

OPA1 Antibody (D-9) References:

1. Nuclear gene OPA1, encoding a mitochondrial dynamin-related protein, is mutated in dominant optic atrophy.  |  Delettre, C., et al. 2000. Nat Genet. 26: 207-10. PMID: 11017079
2. Spectrum, frequency and penetrance of OPA1 mutations in dominant optic atrophy.  |  Toomes, C., et al. 2001. Hum Mol Genet. 10: 1369-78. PMID: 11440989
3. Mutation spectrum and splicing variants in the OPA1 gene.  |  Delettre, C., et al. 2001. Hum Genet. 109: 584-91. PMID: 11810270
4. Differential sublocalization of the dynamin-related protein OPA1 isoforms in mitochondria.  |  Satoh, M., et al. 2003. Biochem Biophys Res Commun. 300: 482-93. PMID: 12504110
5. Roles of the mammalian mitochondrial fission and fusion mediators Fis1, Drp1, and Opa1 in apoptosis.  |  Lee, YJ., et al. 2004. Mol Biol Cell. 15: 5001-11. PMID: 15356267
6. OPA1 requires mitofusin 1 to promote mitochondrial fusion.  |  Cipolat, S., et al. 2004. Proc Natl Acad Sci U S A. 101: 15927-32. PMID: 15509649
7. OPA1, associated with autosomal dominant optic atrophy, is widely expressed in the human brain.  |  Bette, S., et al. 2005. Acta Neuropathol. 109: 393-9. PMID: 15700187
8. Refinement of the dominant optic atrophy locus (OPA1) to a 1.4-cM interval on chromosome 3q28-3q29, within a 3-Mb YAC contig.  |  Jonasdottir, A., et al. 1997. Hum Genet. 99: 115-20. PMID: 9003507

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-OPA1 Antibody (D-9) has 62 citations in a variety of scientific publications.

Primary antibodies like Anti-OPA1 Antibody (D-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

OPA1 Antibody (D-9) Specifications

Alternate Product Name: OPA1 Mitochondrial Dynamin Like GTPase

Product Clone Name: D-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 647-780

Epitope Target: OPA1

Epitope Species: human

Product Reactivity/Detection: OPA1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 62 citations in various scientific publications

Available Conjugates/Forms for OPA1 Antibody (D-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin