Occludin Antibody (F-11): sc-133255

Occludin Antibody (F-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects Occludin of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-Occludin antibody (F-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Occludin is an integral membrane protein that plays a crucial role in forming and maintaining tight junctions in epithelial and endothelial cells, which regulate paracellular permeability and maintain blood-brain barrier integrity. Occludin features a tetraspan structure with two extracellular loops that facilitate interactions with other tight junction proteins. Post-translational modifications, particularly phosphorylation, significantly influence Occludin′s localization and functional properties in tight junction dynamics by modulating interactions with other junctional components and affecting epithelial barrier permeability. Polyunsaturated fatty acids up-regulate Occludin expression, enhancing transendothelial cell resistance and reducing permeability to larger molecules, thus maintaining cellular homeostasis. Occludin expression varies across tissues, showing high levels at cell-cell contact sites in brain tissue while displaying lower and more discontinuous distribution patterns in non-neural tissues. Understanding Occludin′s function and regulation provides crucial insights into various physiological and pathological processes, including inflammation and tumor progression.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Occludin Antibody (F-11) has 25 citations in a variety of scientific publications.

Primary antibodies like Anti-Occludin Antibody (F-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Occludin Antibody (F-11) Specifications

Alternate Product Name: Occludin

Product Clone Name: F-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 132-411

Epitope Target: Occludin

Epitope Species: human

Product Reactivity/Detection: Occludin

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 25 citations in various scientific publications

Available Conjugates/Forms for Occludin Antibody (F-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Occludin antibody is Occludin Antibody (E-5): sc-133256.