Occludin Antibody (E-5) recommended for detection of Occludin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Occludin Antibody (E-5): sc-133256

Occludin Antibody (E-5) is rated 4.6 out of 5 by 29.
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Datasheets

    • Anti-Occludin Antibody (E-5) is a mouse monoclonal IgG2b (kappa light chain) Occludin antibody provided at 200 µg/ml
    • raised against amino acids 132-411 mapping within an internal region of Occludin of human origin
    • Anti-Occludin Antibody (E-5) is recommended for detection of Occludin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Occludin Antibody (E-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Occludin (E-5): sc-133256.
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Am I amble to sample the antibody before I order it?

Asked by: jrauchut
Thank you for your question. Yes, for a limited time we are offering free 10 µg samples of this mouse monoclonal, Occludin Antibody (E-5): sc-133256. Please contact our Technical Service Department to request a free sample. Please call us toll-free at (800) 457-3801 option 2, e-mail <[email protected]> or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-05-04

Can Occludin (E-5): sc-133256 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, Occludin (E-5): sc-133256 monoclonal antibody has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 10
Date published: 2017-01-12

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with Occludin (E-5): sc-133256?

Asked by: jenni
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 10
Date published: 2017-01-12
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Rated 5 out of 5 by from Great product!! It's a great product for me to analyze Western blotting.
Date published: 2018-04-22
Rated 5 out of 5 by from Great antibody Used this to for staining on mouse intestine. Strong signal with minimum background. Works great. Highly recommended.
Date published: 2018-03-26
Rated 3 out of 5 by from Good antibody Used it for staining MDCK cells. Works OK. has some background. Doesnt work for WB
Date published: 2018-01-31
Rated 4 out of 5 by from Good antibody in IF at 1:50. It's OK to test cell tight junction in IF at 1:50.
Date published: 2017-11-23
Rated 4 out of 5 by from Works for WB I got a free sample of this antibody from Santa Cruz earlier with some other junctional proteins. This one showed a clear band in WB close to the predicted molecular weight.
Date published: 2017-11-06
Rated 3 out of 5 by from Good antibody Worked well with western blotting at 1:500 dilution.
Date published: 2017-09-27
Rated 5 out of 5 by from Excellent results by WB on rat brain We have got excellent results by WB on rat brain tissue 1:200
Date published: 2017-09-27
Rated 5 out of 5 by from 申请免费抗体试用装 我之前购买过 santa cruz 家的siRNA 特别好用。之前还购买过 santa cruz 家的大包装的抗体,特别好用。这次希望申请occludin的抗体使用装,条带出就接着购买大包装抗体。
Date published: 2017-09-24
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