OAS1 Antibody (F-3): sc-374656

OAS1 Antibody (F-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects OAS1 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). OAS1 (F-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The 2′-, 5′-oligoadenylate synthetases (OASs) are crucial interferon-induced proteins that play a significant role in the cellular response to viral infections, as well as in the regulation of cell growth, differentiation, and apoptosis. OAS1, functioning as a homotetramer, is particularly notable for catalyzing the synthesis of 2′-, 5′-oligomers of adenosine (2-5As). OAS1 binds to double-stranded RNA and polymerizes ATP into PPP(A2′P5′A)N oligomers, which in turn activate latent RNase L. Once activated, RNase L cleaves single-stranded RNAs, leading to the inhibition of cellular protein synthesis and effectively impairing viral replication. Additionally, OAS1, a protein composed of 400 amino acids, is essential for evaluating the interferon response in RNA interference studies and has been implicated in susceptibility to diabetes mellitus. anti-OAS1 antibody (F-3) serves as a valuable tool for researchers studying viral resistance mechanisms and cellular responses to stress.

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OAS1 Antibody (F-3) References:

1. A specific isozyme of 2'-5' oligoadenylate synthetase is a dual function proapoptotic protein of the Bcl-2 family.  |  Ghosh, A., et al. 2001. J Biol Chem. 276: 25447-55. PMID: 11323417
2. Characterization of the 2'-5'-oligoadenylate synthetase ubiquitin-like family.  |  Eskildsen, S., et al. 2003. Nucleic Acids Res. 31: 3166-73. PMID: 12799444
3. Variation in antiviral 2',5'-oligoadenylate synthetase (2'5'AS) enzyme activity is controlled by a single-nucleotide polymorphism at a splice-acceptor site in the OAS1 gene.  |  Bonnevie-Nielsen, V., et al. 2005. Am J Hum Genet. 76: 623-33. PMID: 15732009
4. Polymorphisms of interferon-inducible genes OAS-1 and MxA associated with SARS in the Vietnamese population.  |  Hamano, E., et al. 2005. Biochem Biophys Res Commun. 329: 1234-9. PMID: 15766558
5. OAS1 splice site polymorphism controlling antiviral enzyme activity influences susceptibility to type 1 diabetes.  |  Field, LL., et al. 2005. Diabetes. 54: 1588-91. PMID: 15855350
6. Enhancing and confirming the specificity of RNAi experiments.  |  Cullen, BR. 2006. Nat Methods. 3: 677-81. PMID: 16929311
7. Heterozygous OAS1 gain-of-function variants cause an autoinflammatory immunodeficiency.  |  Magg, T., et al. 2021. Sci Immunol. 6: PMID: 34145065
8. OAS1 suppresses African swine fever virus replication by recruiting TRIM21 to degrade viral major capsid protein.  |  Sun, H., et al. 2023. J Virol. 97: e0121723. PMID: 37815352
9. Multi-omics analysis reveals interferon-stimulated gene OAS1 as a prognostic and immunological biomarker in pan-cancer.  |  Yang, R., et al. 2023. Front Immunol. 14: 1249731. PMID: 37928544
10. Induction of 2.5 OAS gene expression and activity is not sufficient for IFN-gamma-induced neuroblastoma cell differentiation.  |  Corrias, MV., et al. 1995. Int J Cancer. 62: 223-9. PMID: 7622300
11. The human 2',5'-oligoadenylate synthetase locus is composed of three distinct genes clustered on chromosome 12q24.2 encoding the 100-, 69-, and 40-kDa forms.  |  Hovnanian, A., et al. 1998. Genomics. 52: 267-77. PMID: 9790745
12. Structure of two forms of the interferon-induced (2'-5') oligo A synthetase of human cells based on cDNAs and gene sequences.  |  Benech, P., et al. 1985. EMBO J. 4: 2249-56. PMID: 2416561

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-OAS1 Antibody (F-3) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-OAS1 Antibody (F-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

OAS1 Antibody (F-3) Specifications

Alternate Product Name: OAS1

Product Clone Name: F-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 337-364

Epitope Target: OAS1 p41

Epitope Species: human

Product Reactivity/Detection: OAS1 p41 isoform

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for OAS1 Antibody (F-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent