Nup153 Antibody (D-4): sc-515373

Nup153 Antibody (D-4) is a mouse monoclonal IgG2b antibody that detects Nup153 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Nup153 plays a crucial role in the assembly and function of nuclear pore complexes (NPCs), which are essential for the regulated transport of macromolecules between the nucleus and cytoplasm. Nup153 is strategically located at the periphery of NPCs, where Nup153 facilitates the interaction between the nuclear pore and the nuclear lamina, thereby contributing to the structural integrity of the nuclear envelope. The unique structural features of Nup153, including its N-terminal region, central domain with multiple zinc fingers, and a C-terminal region rich in FXFG repeats, are vital for mediating specific nuclear import events and anchoring NPCs. Additionally, Nup153′s interaction with the mRNA export factor TAP underscores Nup153′s importance in the transport of RNA-protein complexes, which is critical for gene expression regulation. Nup153′s ability to undergo cleavage by caspases during apoptosis further highlights Nup153′s significance in cellular processes, making anti-Nup153 antibody (D-4) an invaluable tool for researchers studying nuclear transport mechanisms and their implications in various biological contexts.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Nup153 Antibody (D-4) References:

1. The nucleoporin nup153 plays a critical role in multiple types of nuclear export.  |  Ullman, KS., et al. 1999. Mol Biol Cell. 10: 649-64. PMID: 10069809
2. RNA association defines a functionally conserved domain in the nuclear pore protein Nup153.  |  Dimaano, C., et al. 2001. J Biol Chem. 276: 45349-57. PMID: 11567018
3. The nucleoporin Nup153 affects spindle checkpoint activity due to an association with Mad1.  |  Lussi, YC., et al. 2010. Nucleus. 1: 71-84. PMID: 21327106
4. Nucleoporin NUP153 guards genome integrity by promoting nuclear import of 53BP1.  |  Moudry, P., et al. 2012. Cell Death Differ. 19: 798-807. PMID: 22075984
5. Nup153 and Nup98 bind the HIV-1 core and contribute to the early steps of HIV-1 replication.  |  Di Nunzio, F., et al. 2013. Virology. 440: 8-18. PMID: 23523133
6. Localisation of Nup153 and SENP1 to nuclear pore complexes is required for 53BP1-mediated DNA double-strand break repair.  |  Duheron, V., et al. 2017. J Cell Sci. 130: 2306-2316. PMID: 28576968
7. Altered Nup153 Expression Impairs the Function of Cultured Hippocampal Neural Stem Cells Isolated from a Mouse Model of Alzheimer's Disease.  |  Leone, L., et al. 2019. Mol Neurobiol. 56: 5934-5949. PMID: 30689197
8. Mitotic checkpoint protein Mad1 is required for early Nup153 recruitment to chromatin and nuclear envelope integrity.  |  Mossaid, I., et al. 2020. J Cell Sci. 133: PMID: 33023979
9. A role for Nup153 in nuclear assembly reveals differential requirements for targeting of nuclear envelope constituents.  |  LaJoie, D., et al. 2022. Mol Biol Cell. 33: ar117. PMID: 36044344
10. Human RTEL1 Interacts with KPNB1 (Importin β) and NUP153 and Connects Nuclear Import to Nuclear Envelope Stability in S-Phase.  |  Schertzer, M., et al. 2023. Cells. 12: PMID: 38132118

The preferred Nup153 antibody is Nup153 Antibody (R3G1): sc-101544.