Nup153 Antibody (R3G1): sc-101544

Nup153 Antibody (R3G1) is a rat monoclonal IgG2a antibody that detects Nup153 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Nup153 antibody (R3G1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Nup153 plays a critical role in the structure and function of nuclear pore complexes (NPCs), which are essential for the regulated transport of macromolecules between the nucleus and cytoplasm. Nup153 is a peripheral component of NPCs and is characterized by a unique N-terminal region, a central domain with four to five zinc fingers, and a C-terminal region rich in FXFG repeats. This structural organization is vital for its function in anchoring NPCs within the nuclear envelope and facilitating specific nuclear import events. Additionally, Nup153 is involved in the assembly of NPCs and interacts with key proteins such as TAP, which is crucial for mRNA export. The presence of O-linked N-acetylglucosamine moieties and the distinctive pentapeptide repeat motif (XFXFG) in nucleoporins, including Nup153, underscores the importance of post-translational modifications in regulating NPC dynamics and function. Nup153′s cleavage by caspases during apoptosis further highlights its significance in cellular processes, making it a valuable target for research in nuclear transport and cell cycle regulation.

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Nup153 Antibody (R3G1) References:

1. Sequential recruitment of NPC proteins to the nuclear periphery at the end of mitosis.  |  Bodoor, K., et al. 1999. J Cell Sci. 112 (Pt 13): 2253-64. PMID: 10362555
2. The mRNA export in Caenorhabditis elegans is mediated by Ce-NXF-1, an ortholog of human TAP/NXF and Saccharomyces cerevisiae Mex67p.  |  Tan, W., et al. 2000. RNA. 6: 1762-72. PMID: 11142376
3. Caspases mediate nucleoporin cleavage, but not early redistribution of nuclear transport factors and modulation of nuclear permeability in apoptosis.  |  Ferrando-May, E., et al. 2001. Cell Death Differ. 8: 495-505. PMID: 11423910
4. The nucleoporin Nup153 is required for nuclear pore basket formation, nuclear pore complex anchoring and import of a subset of nuclear proteins.  |  Walther, TC., et al. 2001. EMBO J. 20: 5703-14. PMID: 11598013
5. Nucleoporin NUP153 phenylalanine-glycine motifs engage a common binding pocket within the HIV-1 capsid protein to mediate lentiviral infectivity.  |  Matreyek, KA., et al. 2013. PLoS Pathog. 9: e1003693. PMID: 24130490
6. NUP153 overexpression suppresses the proliferation of colorectal cancer by negatively regulating Wnt/β-catenin signaling pathway and predicts good prognosis.  |  Wu, Y., et al. 2019. Cancer Biomark. 24: 61-70. PMID: 30347601
7. NUP153 promotes HCC cells proliferation via c-Myc-mediated downregulation of P15INK4b.  |  Gan, C., et al. 2022. Dig Liver Dis. 54: 1706-1715. PMID: 35288064
8. The capsid lattice engages a bipartite NUP153 motif to mediate nuclear entry of HIV-1 cores.  |  Shen, Q., et al. 2023. Proc Natl Acad Sci U S A. 120: e2202815120. PMID: 36943880
9. Nup153 is not required for anchoring heterochromatic DSBs to the nuclear periphery.  |  Ryu, T., et al. 2024. MicroPubl Biol. 2024: PMID: 38737725
10. Cul3 substrate adaptor SPOP targets Nup153 for degradation.  |  Ong, JY., et al. 2025. Mol Biol Cell. 36: ar24. PMID: 39785820
11. Sequence analysis of a cDNA encoding a human nuclear pore complex protein, hnup153.  |  McMorrow, I., et al. 1994. Biochim Biophys Acta. 1217: 219-23. PMID: 8110839
12. Targeting and function in mRNA export of nuclear pore complex protein Nup153.  |  Bastos, R., et al. 1996. J Cell Biol. 134: 1141-56. PMID: 8794857

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Nup153 Antibody (R3G1) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-Nup153 Antibody (R3G1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Nup153 Antibody (R3G1) Specifications

Alternate Product Name: Nup153

Product Clone Name: R3G1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a

Amino Acids: 610-1191

Epitope Target: Nup153

Epitope Species: rat

Product Reactivity/Detection: Nup153

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for Nup153 Antibody (R3G1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin