NHE-3 Antibody (53): sc-136368

NHE-3 Antibody (53) is a mouse monoclonal IgG1 kappa light chain antibody that detects NHE-3 protein of mouse, rat, and human origin by western blotting (WB) and immunoprecipitation (IP). Anti-NHE-3 antibody (53) is available in both non-conjugated and various conjugated forms, including agarose and horseradish peroxidase (HRP). NHE-3 protein plays a crucial role in regulating sodium transport and fluid reabsorption, primarily localizing to the apical membrane of renal proximal tubules. This localization allows NHE-3 to effectively mediate the exchange of sodium ions for hydrogen ions, which is essential for maintaining intracellular pH and cell volume. NHE-3 is involved in the body′s response to changes in blood pressure, as NHE-3 translocates to internal pools to facilitate natriuresis when necessary. Beyond the kidneys, NHE-3 is also expressed in the stomach, where NHE-3 contributes to mucosal protection by secreting protons that diffuse into the mucous cells, highlighting its importance in both renal and gastrointestinal physiology.

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NHE-3 Antibody (53) References:

1. Protective role of NHE-3 inhibition in rat renal transplantation undergoing acute rejection.  |  Reuter, S., et al. 2008. Pflugers Arch. 456: 1075-84. PMID: 18335234
2. Reduced expression of Na⁺/H⁺ exchanger isoform 3 (NHE-3) in preeclamptic placentas.  |  Dietrich, V., et al. 2013. Placenta. 34: 828-30. PMID: 23810111
3. Phosphorylation and subcellular localization of Na+/H+ exchanger isoform 3 (NHE3) are associated with altered gallbladder absorptive function after formation of cholesterol gallstones.  |  Chen, Y., et al. 2017. J Physiol Biochem. 73: 133-139. PMID: 27822917
4. Flupirtine enhances NHE-3-mediated Na+ absorption in rat colon via an ENS-dependent mechanism.  |  Nickerson, AJ. and Rajendran, VM. 2021. Am J Physiol Gastrointest Liver Physiol. 321: G185-G199. PMID: 34132108
5. Structural basis of autoinhibition of the human NHE3-CHP1 complex.  |  Dong, Y., et al. 2022. Sci Adv. 8: eabn3925. PMID: 35613257
6. NHE3 in the thick ascending limb is required for sustained but not acute furosemide-induced urinary acidification.  |  Xue, J., et al. 2022. Am J Physiol Renal Physiol. 323: F141-F155. PMID: 35635321
7. Intestine-Specific NHE3 Deletion in Adulthood Causes Microbial Dysbiosis.  |  Xue, J., et al. 2022. Front Cell Infect Microbiol. 12: 896309. PMID: 35719363
8. Renal upregulation of NCC counteracts empagliflozin-mediated NHE3 inhibition in normotensive but not in hypertensive male rat.  |  Castro, PC., et al. 2024. Am J Physiol Cell Physiol. 326: C1573-C1589. PMID: 38557357
9. SGLT2-independent effects of canagliflozin on NHE3 and mitochondrial complex I activity inhibit proximal tubule fluid transport and albumin uptake.  |  Albalawy, WN., et al. 2024. Am J Physiol Renal Physiol. 326: F1041-F1053. PMID: 38660713
10. Genetic deletion of the kidney sodium/proton exchanger-3 (NHE3) does not alter calcium and phosphate balance due to compensatory responses.  |  Poulsen, SB., et al. 2025. Kidney Int. 107: 280-295. PMID: 39089578

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NHE-3 Antibody (53) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-NHE-3 Antibody (53) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NHE-3 Antibody (53) Specifications

Alternate Product Name: NHE-3

Product Clone Name: 53

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 725-831

Epitope Target: NHE-3

Epitope Species: rat

Product Reactivity/Detection: NHE-3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for NHE-3 Antibody (53): AC: Agarose, HRP: Horseradish Peroxidase, X: TransCruz Reagent

The preferred p-NHE-3 antibody is p-NHE-3 Antibody (14D5): sc-53962.