NHE-1 Antibody (54): sc-136239

NHE-1 Antibody (54) is a mouse monoclonal IgG1 kappa light chain antibody that detects NHE-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-NHE-1 antibody (54) is available in both non-conjugated and various conjugated forms, including agarose and horseradish peroxidase (HRP). NHE-1, also known as solute carrier family 9 isoform-1 (SLC9A1), plays a crucial role in maintaining cellular homeostasis by regulating intracellular pH and cell volume through the exchange of sodium (Na+) and hydrogen (H+) ions across the plasma membrane. NHE-1 is predominantly located in the plasma membrane of various mammalian tissues, where NHE-1 functions as a vital membrane-associated pH regulator. Proper functioning of NHE-1 is essential for numerous physiological processes, including cell proliferation, migration, and apoptosis, as NHE-1 helps prevent cellular acidosis and maintain optimal conditions for enzymatic activities. Additionally, NHE-1 is characterized by ten transmembrane domains, an N-terminal amphipathic domain, and two potential N-glycosylation sites, which are important for stability and function. These unique structural features of NHE-1 not only facilitate its role as a sodium-hydrogen exchanger but also influence interaction with other proteins and signaling pathways, making NHE-1 a significant target for research in various fields, including cardiovascular and renal physiology.

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NHE-1 Antibody (54) References:

1. Direct binding of the Na--H exchanger NHE1 to ERM proteins regulates the cortical cytoskeleton and cell shape independently of H(+) translocation.  |  Denker, SP., et al. 2000. Mol Cell. 6: 1425-36. PMID: 11163215
2. NHE-1 inhibition: from protection during acute ischaemia/reperfusion to prevention/reversal of myocardial remodelling.  |  Linz, WJ. and Busch, AE. 2003. Naunyn Schmiedebergs Arch Pharmacol. 368: 239-46. PMID: 14504689
3. NHE-1 and NHE-6 activities: ischemic and reperfusion injury.  |  Cingolani, HE., et al. 2003. Circ Res. 93: 694-6. PMID: 14563708
4. Molecular cloning of putative members of the Na/H exchanger gene family. cDNA cloning, deduced amino acid sequence, and mRNA tissue expression of the rat Na/H exchanger NHE-1 and two structurally related proteins.  |  Orlowski, J., et al. 1992. J Biol Chem. 267: 9331-9. PMID: 1577762
5. The role of NHE-1 in myocardial hypertrophy and remodelling.  |  Karmazyn, M., et al. 2008. J Mol Cell Cardiol. 44: 647-53. PMID: 18329039
6. NHE-1: a molecular target for signalling and cell matrix interactions.  |  Koliakos, G., et al. 2008. Connect Tissue Res. 49: 157-61. PMID: 18661333
7. Empagliflozin mitigates cardiac hypertrophy through cardiac RSK/NHE-1 inhibition.  |  Chen, S., et al. 2024. Biomed Pharmacother. 174: 116477. PMID: 38522235
8. Molecular cloning and physical and genetic mapping of a novel human Na+/H+ exchanger (NHE5/SLC9A5) to chromosome 16q22.1.  |  Klanke, CA., et al. 1995. Genomics. 25: 615-22. PMID: 7759094
9. Hormonal regulation, pharmacology, and membrane sorting of vertebrate Na+/H+ exchanger isoforms.  |  Noël, J. and Pouysségur, J. 1995. Am J Physiol. 268: C283-96. PMID: 7864067
10. NHE3: a Na+/H+ exchanger isoform of renal brush border.  |  Biemesderfer, D., et al. 1993. Am J Physiol. 265: F736-42. PMID: 8238556
11. Cloning and analysis of the human myocardial Na+/H+ exchanger.  |  Fliegel, L., et al. 1993. Mol Cell Biochem. 125: 137-43. PMID: 8283968

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NHE-1 Antibody (54) has 48 citations in a variety of scientific publications.

Primary antibodies like Anti-NHE-1 Antibody (54) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NHE-1 Antibody (54) Specifications

Alternate Product Name: SLC9A1

Product Clone Name: 54

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 682-801

Epitope Target: NHE-1

Epitope Species: rat

Product Reactivity/Detection: NHE-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 48 citations in various scientific publications

Available Conjugates/Forms for NHE-1 Antibody (54): AC: Agarose, HRP: Horseradish Peroxidase