NFATc3 Antibody (F-1): sc-8405

NFATc3 Antibody (F-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects NFATc3 of mouse, rat, and human origin through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHC), and enzyme-linked immunosorbent assay (ELISA). NFATc3 monoclonal antibody (F-1) is offered in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and various Alexa Fluor® conjugates. NFATc3, also known as nuclear factor of activated T cells 3, plays a crucial role in the immune response by regulating the expression of cytokines in T cells. NFATc3 is part of the NFAT family of transcription factors, which are activated by increases in intracellular calcium levels. This activation leads to the dephosphorylation of NFAT proteins by the calmodulin-dependent phosphatase calcineurin, resulting in a conformational change that exposes the nuclear localization signal. This change is vital as NFAT proteins translocate from the cytosol into the nucleus, where these proteins bind to DNA and modulate gene expression. NFATc3 interaction with other proteins, such as AP-1 proteins (Fos and Jun), further underscores NFATc3′s importance in forming cooperative complexes that regulate immune responses.

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NFATc3 Antibody (F-1) References:

1. Recognition of NFATp/AP-1 composite elements within genes induced upon the activation of immune cells.  |  Kel, A., et al. 1999. J Mol Biol. 288: 353-76. PMID: 10329147
2. NFAT5, a constitutively nuclear NFAT protein that does not cooperate with Fos and Jun.  |  Lopez-Rodríguez, C., et al. 1999. Proc Natl Acad Sci U S A. 96: 7214-9. PMID: 10377394
3. Enhanced NFATc1 nuclear occupancy causes T cell activation independent of CD28 costimulation.  |  Pan, M., et al. 2007. J Immunol. 178: 4315-21. PMID: 17371988
4. Macrophage NFATc3 prevents foam cell formation and atherosclerosis: evidence and mechanisms.  |  Liu, X., et al. 2021. Eur Heart J. 42: 4847-4861. PMID: 34570211
5. Trim39 regulates neuronal apoptosis by acting as a SUMO-targeted E3 ubiquitin-ligase for the transcription factor NFATc3.  |  Basu-Shrivastava, M., et al. 2022. Cell Death Differ. 29: 2107-2122. PMID: 35449213
6. NFATc3 Promotes Pulmonary Inflammation and Fibrosis by Regulating Production of CCL2 and CXCL2 in Macrophages.  |  Nie, Y., et al. 2023. Aging Dis. 14: 1441-1457. PMID: 37523510
7. Isolation of two new members of the NF-AT gene family and functional characterization of the NF-AT proteins.  |  Hoey, T., et al. 1995. Immunity. 2: 461-72. PMID: 7749981
8. Characterization of a new isoform of the NFAT (nuclear factor of activated T cells) gene family member NFATc.  |  Park, J., et al. 1996. J Biol Chem. 271: 20914-21. PMID: 8702849
9. Expression of NFAT-family proteins in normal human T cells.  |  Lyakh, L., et al. 1997. Mol Cell Biol. 17: 2475-84. PMID: 9111316
10. Transcription factors of the NFAT family: regulation and function.  |  Rao, A., et al. 1997. Annu Rev Immunol. 15: 707-47. PMID: 9143705
11. Distinct NFAT family proteins are involved in the nuclear NFAT-DNA binding complexes from human thymocyte subsets.  |  Amasaki, Y., et al. 1998. J Immunol. 160: 2324-33. PMID: 9498773
12. The transcription factor NF-ATc is essential for cardiac valve formation.  |  Ranger, AM., et al. 1998. Nature. 392: 186-90. PMID: 9515964

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NFATc3 Antibody (F-1) has 101 citations in a variety of scientific publications.

Primary antibodies like Anti-NFATc3 Antibody (F-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NFATc3 Antibody (F-1) Specifications

Alternate Product Name: NFATc3

Product Clone Name: F-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 321-395

Epitope Target: NFATc3

Epitope Species: mouse

Product Reactivity/Detection: NFATc3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 101 citations in various scientific publications

Available Conjugates/Forms for NFATc3 Antibody (F-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent

The preferred p-NFATc3 antibody is p-NFATc3 Antibody (H-2): sc-365883.