nephrin Antibody (E-1): sc-376568

Nephrin Antibody (E-1) is a mouse monoclonal IgG2b antibody that detects nephrin in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Nephrin is a crucial component of the glomerular filtration barrier, primarily located in the podocytes of the kidney, where nephrin plays a vital role in maintaining the selective permeability of the glomerular slit diaphragm. Nephrin is essential for preventing the passage of large molecules, such as proteins, into the urine, thereby ensuring proper kidney function and homeostasis. The nephrin gene, located on chromosome 19q13.1, encodes a 1,241 amino acid protein featuring a transmembrane domain, eight immunoglobulin-like domains, and one fibronectin III-like domain, which collectively contribute to structural integrity and function. Nephrin′s interaction with podocin enhances signaling capabilities, particularly in activating mitogen-activated protein kinases, which are critical for cellular responses to various stimuli. Additionally, nephrin expression can be upregulated by protein kinase C signaling pathways, indicating involvement in complex intracellular signaling networks that regulate kidney function. Anti-nephrin antibody (E-1) is an invaluable tool for researchers studying kidney physiology and pathophysiology, particularly in conditions such as nephrotic syndrome, where nephrin function is compromised.

The preferred nephrin antibody is nephrin Antibody (B-12): sc-377246.