nephrin Antibody (B-12): sc-377246
- nephrin Antibody (B-12) is a mouse monoclonal IgG2a κ, cited in 30 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 55-93 near the N-terminus of nephrin of human origin
- Anti-nephrin Antibody (B-12) is recommended for detection of nephrin of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-nephrin Antibody (B-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for nephrin Antibody (B-12) for WB applications. These reagents are now offered in bundles with nephrin Antibody (B-12) (see ordering information below).
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SEE ALSO...
nephrin Antibody (B-12) is a mouse monoclonal IgG2a kappa light chain antibody that detects nephrin in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-nephrin antibody (B-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. nephrin plays a crucial role in the kidney′s filtration barrier, as nephrin is a key component of the slit diaphragm formed between podocytes, specialized epithelial cells that regulate the filtration of blood. nephrin′s structure includes a transmembrane domain, eight immunoglobulin-like modules, and one fibronectin III-like module, which are essential for function in cell adhesion and signaling. The nephrin gene is located on chromosome 19q13.1 and encodes a 1,241 amino acid protein that is vital for maintaining the integrity of the glomerular filtration barrier. nephrin′s interaction with podocin enhances signaling capabilities, particularly in activating mitogen-activated protein kinases, which are important for cellular responses. Additionally, nephrin expression can be upregulated in A293 cells treated with Phorbol-12-myristate-13-acetate, indicating that protein kinase C is involved in the intracellular signaling pathways that regulate nephrin activity.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
nephrin Antibody (B-12) References:
- Nephrin localizes to the slit pore of the glomerular epithelial cell. | Holzman, LB., et al. 1999. Kidney Int. 56: 1481-91. PMID: 10504499
- Nephrin mRNA regulation by protein kinase C. | Wang, SX., et al. 2001. J Nephrol. 14: 98-103. PMID: 11411021
- Nephrin is an important component of the barrier system in the testis. | Liu, L., et al. 2001. Acta Med Okayama. 55: 161-5. PMID: 11434428
- Involvement of lipid rafts in nephrin phosphorylation and organization of the glomerular slit diaphragm. | Simons, M., et al. 2001. Am J Pathol. 159: 1069-77. PMID: 11549599
- Interaction with podocin facilitates nephrin signaling. | Huber, TB., et al. 2001. J Biol Chem. 276: 41543-6. PMID: 11562357
- Role of nephrin in renal disease including diabetic nephropathy. | Cooper, ME., et al. 2002. Semin Nephrol. 22: 393-8. PMID: 12224046
- Nephrin Preserves Podocyte Viability and Glomerular Structure and Function in Adult Kidneys. | Li, X., et al. 2015. J Am Soc Nephrol. 26: 2361-77. PMID: 25644109
- Endoplasmic reticulum-associated degradation is required for nephrin maturation and kidney glomerular filtration function. | Yoshida, S., et al. 2021. J Clin Invest. 131: PMID: 33591954
- Impaired NEPHRIN localization in kidney organoids derived from nephrotic patient iPS cells. | Ohmori, T., et al. 2021. Sci Rep. 11: 3982. PMID: 33597637
- Morphological analyses of nephrin expression in progressive glomerulonephropathy of common marmosets. | Yamada, N., et al. 2021. J Toxicol Pathol. 34: 83-88. PMID: 33627947
- Nephrin-Ephrin-B1-Na+/H+ Exchanger Regulatory Factor 2-Ezrin-Actin Axis Is Critical in Podocyte Injury. | Fukusumi, Y., et al. 2021. Am J Pathol. 191: 1209-1226. PMID: 33887216
- The Point-Of-Care Test to Detect Nephrin from Urine in Preeclampsia. | Lee, KN., et al. 2023. Clin Lab. 69: PMID: 36649524
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
nephrin Antibody (B-12) | sc-377246 | 200 µg/ml | $322.00 | |||
nephrin Antibody (B-12): m-IgG Fc BP-HRP Bundle | sc-529995 | 200 µg Ab; 10 µg BP | $361.00 | |||
nephrin Antibody (B-12): m-IgGκ BP-HRP Bundle | sc-523248 | 200 µg Ab, 40 µg BP | $361.00 | |||
nephrin Antibody (B-12): m-IgG2a BP-HRP Bundle | sc-547415 | 200 µg Ab; 10 µg BP | $361.00 | |||
nephrin Antibody (B-12) AC | sc-377246 AC | 500 µg/ml, 25% agarose | $424.00 | |||
nephrin Antibody (B-12) HRP | sc-377246 HRP | 200 µg/ml | $322.00 | |||
nephrin Antibody (B-12) FITC | sc-377246 FITC | 200 µg/ml | $336.00 | |||
nephrin Antibody (B-12) PE | sc-377246 PE | 200 µg/ml | $349.00 | |||
nephrin Antibody (B-12) Alexa Fluor® 488 | sc-377246 AF488 | 200 µg/ml | $364.00 | |||
nephrin Antibody (B-12) Alexa Fluor® 546 | sc-377246 AF546 | 200 µg/ml | $364.00 | |||
nephrin Antibody (B-12) Alexa Fluor® 594 | sc-377246 AF594 | 200 µg/ml | $364.00 | |||
nephrin Antibody (B-12) Alexa Fluor® 647 | sc-377246 AF647 | 200 µg/ml | $364.00 | |||
nephrin Antibody (B-12) Alexa Fluor® 680 | sc-377246 AF680 | 200 µg/ml | $364.00 | |||
nephrin Antibody (B-12) Alexa Fluor® 790 | sc-377246 AF790 | 200 µg/ml | $364.00 | |||
nephrin (B-12) Neutralizing Peptide | sc-377246 P | 100 µg/0.5 ml | $69.00 |