nephrin Antibody (B-12): sc-377246

nephrin Antibody (B-12) is a mouse monoclonal IgG2a kappa light chain antibody that detects nephrin in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-nephrin antibody (B-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. nephrin plays a crucial role in the kidney′s filtration barrier, as nephrin is a key component of the slit diaphragm formed between podocytes, specialized epithelial cells that regulate the filtration of blood. nephrin′s structure includes a transmembrane domain, eight immunoglobulin-like modules, and one fibronectin III-like module, which are essential for function in cell adhesion and signaling. The nephrin gene is located on chromosome 19q13.1 and encodes a 1,241 amino acid protein that is vital for maintaining the integrity of the glomerular filtration barrier. nephrin′s interaction with podocin enhances signaling capabilities, particularly in activating mitogen-activated protein kinases, which are important for cellular responses. Additionally, nephrin expression can be upregulated in A293 cells treated with Phorbol-12-myristate-13-acetate, indicating that protein kinase C is involved in the intracellular signaling pathways that regulate nephrin activity.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

nephrin Antibody (B-12) References:

1. Nephrin localizes to the slit pore of the glomerular epithelial cell.  |  Holzman, LB., et al. 1999. Kidney Int. 56: 1481-91. PMID: 10504499
2. Nephrin mRNA regulation by protein kinase C.  |  Wang, SX., et al. 2001. J Nephrol. 14: 98-103. PMID: 11411021
3. Nephrin is an important component of the barrier system in the testis.  |  Liu, L., et al. 2001. Acta Med Okayama. 55: 161-5. PMID: 11434428
4. Involvement of lipid rafts in nephrin phosphorylation and organization of the glomerular slit diaphragm.  |  Simons, M., et al. 2001. Am J Pathol. 159: 1069-77. PMID: 11549599
5. Interaction with podocin facilitates nephrin signaling.  |  Huber, TB., et al. 2001. J Biol Chem. 276: 41543-6. PMID: 11562357
6. Role of nephrin in renal disease including diabetic nephropathy.  |  Cooper, ME., et al. 2002. Semin Nephrol. 22: 393-8. PMID: 12224046
7. Nephrin Preserves Podocyte Viability and Glomerular Structure and Function in Adult Kidneys.  |  Li, X., et al. 2015. J Am Soc Nephrol. 26: 2361-77. PMID: 25644109
8. Endoplasmic reticulum-associated degradation is required for nephrin maturation and kidney glomerular filtration function.  |  Yoshida, S., et al. 2021. J Clin Invest. 131: PMID: 33591954
9. Impaired NEPHRIN localization in kidney organoids derived from nephrotic patient iPS cells.  |  Ohmori, T., et al. 2021. Sci Rep. 11: 3982. PMID: 33597637
10. Morphological analyses of nephrin expression in progressive glomerulonephropathy of common marmosets.  |  Yamada, N., et al. 2021. J Toxicol Pathol. 34: 83-88. PMID: 33627947
11. Nephrin-Ephrin-B1-Na+/H+ Exchanger Regulatory Factor 2-Ezrin-Actin Axis Is Critical in Podocyte Injury.  |  Fukusumi, Y., et al. 2021. Am J Pathol. 191: 1209-1226. PMID: 33887216
12. The Point-Of-Care Test to Detect Nephrin from Urine in Preeclampsia.  |  Lee, KN., et al. 2023. Clin Lab. 69: PMID: 36649524

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-nephrin Antibody (B-12) has 30 citations in a variety of scientific publications.

Primary antibodies like Anti-nephrin Antibody (B-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

nephrin Antibody (B-12) Specifications

Alternate Product Name: NPHS1

Product Clone Name: B-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 55-93

Epitope Target: nephrin

Epitope Species: human

Product Reactivity/Detection: nephrin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 30 citations in various scientific publications

Available Conjugates/Forms for nephrin Antibody (B-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin