NELF-D Antibody (E-8): sc-393682

NELF-D Antibody (E-8) is a mouse monoclonal IgG1 antibody that detects NELF-D in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NELF-D, also known as TH1, NELF-C, HSPC130, or TH1L, is a 590 amino acid protein that plays a crucial role in regulating transcription elongation by negatively influencing RNA polymerase II activity. NELF-D is primarily localized in the nucleus, where NELF-D functions as a key component of the NELF complex, essential for controlling transcriptional pausing. NELF-D′s ability to induce chromatin unfolding is particularly significant, as this function may contribute to gene expression regulation in various physiological and pathological contexts, including tumorigenesis. NELF-D is encoded by a gene located on human chromosome 20, a chromosome comprising about 2% of the human genome and containing over 600 genes associated with various diseases, including neurodegenerative disorders and syndromes affecting reproductive health. NELF-D expression across multiple tissues, including liver, heart, kidney, lung, brain, placenta, and pancreas, underscores NELF-D′s importance in cellular function and development. Anti-NELF-D antibody (E-8) serves as an invaluable tool for researchers investigating transcription regulation mechanisms and their implications in health and disease.

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NELF-D Antibody (E-8) References:

1. Characterization of hampin/MSL1 as a node in the nuclear interactome.  |  Dmitriev, RI., et al. 2007. Biochem Biophys Res Commun. 355: 1051-7. PMID: 17335777
2. Multiple putative oncogenes at the chromosome 20q amplicon contribute to colorectal adenoma to carcinoma progression.  |  Carvalho, B., et al. 2009. Gut. 58: 79-89. PMID: 18829976
3. The role of prostate specific membrane antigen and pepsinogen C tissue expression as an adjunctive method to prostate cancer diagnosis.  |  Antunes, AA., et al. 2009. J Urol. 181: 594-600. PMID: 19084862
4. GREB1 tissue expression is associated with organ-confined prostate cancer.  |  Antunes, AA., et al. 2012. Urol Oncol. 30: 16-20. PMID: 19945309
5. Integrated analysis of genome-wide copy number alterations and gene expression in microsatellite stable, CpG island methylator phenotype-negative colon cancer.  |  Loo, LW., et al. 2013. Genes Chromosomes Cancer. 52: 450-66. PMID: 23341073
6. An integrative approach identified genes associated with drug response in gastric cancer.  |  Zhou, J., et al. 2015. Carcinogenesis. 36: 441-51. PMID: 25742747
7. Genome-wide analysis of positively selected genes in seasonal and non-seasonal breeding species.  |  Meng, Y., et al. 2015. PLoS One. 10: e0126736. PMID: 26000771
8. NELF complex fosters BRCA1 and RAD51 recruitment to DNA damage sites and modulates sensitivity to PARP inhibition.  |  Bishara, LA., et al. 2021. DNA Repair (Amst). 97: 103025. PMID: 33248388
9. NELF coordinates Pol II transcription termination and DNA replication initiation.  |  Nakayama, C., et al. 2024. bioRxiv.. PMID: 38352431
10. TH1L involvement in colorectal cancer pathogenesis by regulation of CCL20 through the NF-κB signalling pathway.  |  Wang, S., et al. 2024. J Cell Mol Med. 28: e18391. PMID: 38809918

The preferred NELF-D antibody is NELF-D Antibody (C-10): sc-393972.