NELF-D Antibody (C-7): sc-393628

NELF-D Antibody (C-7) is a mouse monoclonal IgG1 antibody that detects NELF-D in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NELF-D, also known as TH1, NELF-C, HSPC130, or TH1L, is a 590 amino acid protein that plays a crucial role in regulating transcription elongation by negatively influencing RNA polymerase II activity. NELF-D is primarily localized in the nucleus, where NELF-D forms part of the multi-protein NELF complex, essential for controlling transcriptional pausing of RNA polymerase II. NELF-D′s ability to induce chromatin unfolding is particularly significant, as NELF-D may contribute to gene expression regulation in various physiological and pathological contexts, including tumorigenesis. NELF-D is encoded by a gene located on human chromosome 20, a chromosome that comprises approximately 2% of the human genome and contains over 600 genes associated with various diseases, including Creutzfeldt-Jakob disease and amyotrophic lateral sclerosis. NELF-D′s diverse expression across multiple tissues, including the liver, heart, kidney, lung, brain, placenta, and pancreas, underscores NELF-D′s importance in cellular function and highlights NELF-D′s potential as a target for therapeutic interventions.

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NELF-D Antibody (C-7) References:

1. Human transcription elongation factor NELF: identification of novel subunits and reconstitution of the functionally active complex.  |  Narita, T., et al. 2003. Mol Cell Biol. 23: 1863-73. PMID: 12612062
2. The role of prostate specific membrane antigen and pepsinogen C tissue expression as an adjunctive method to prostate cancer diagnosis.  |  Antunes, AA., et al. 2009. J Urol. 181: 594-600. PMID: 19084862
3. Trihydrophobin 1 attenuates androgen signal transduction through promoting androgen receptor degradation.  |  Yang, Y., et al. 2010. J Cell Biochem. 109: 1013-24. PMID: 20069563
4. An integrative approach identified genes associated with drug response in gastric cancer.  |  Zhou, J., et al. 2015. Carcinogenesis. 36: 441-51. PMID: 25742747
5. Genome-wide analysis of positively selected genes in seasonal and non-seasonal breeding species.  |  Meng, Y., et al. 2015. PLoS One. 10: e0126736. PMID: 26000771
6. Architecture and RNA binding of the human negative elongation factor.  |  Vos, SM., et al. 2016. Elife. 5: PMID: 27282391
7. NELF complex fosters BRCA1 and RAD51 recruitment to DNA damage sites and modulates sensitivity to PARP inhibition.  |  Bishara, LA., et al. 2021. DNA Repair (Amst). 97: 103025. PMID: 33248388
8. PHRF1 promotes the class switch recombination of IgA in CH12F3-2A cells.  |  Lee, JY., et al. 2023. PLoS One. 18: e0285159. PMID: 37540725
9. NELF coordinates Pol II transcription termination and DNA replication initiation.  |  Nakayama, C., et al. 2024. bioRxiv.. PMID: 38352431
10. TH1L involvement in colorectal cancer pathogenesis by regulation of CCL20 through the NF-κB signalling pathway.  |  Wang, S., et al. 2024. J Cell Mol Med. 28: e18391. PMID: 38809918

The preferred NELF-D antibody is NELF-D Antibody (C-10): sc-393972.