Nectin 3 Antibody (N3.81.6): sc-69716

Nectin 3 Antibody (N3.81.6) is a mouse monoclonal IgG2b antibody that detects Nectin 3 in human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). Nectin 3 is a crucial transmembrane protein that plays a significant role in cell adhesion and communication, particularly in the nervous system and during viral infections. Nectin 3 is part of the Nectin family, which includes Nectin 1, 2, and 3, and is characterized by three immunoglobulin-like domains in the extracellular region. Notably, Nectin 3 has three splicing variants: Nectin 3α, 3β, and 3γ, with the first two containing a conserved C-terminal motif that is essential for binding to the PDZ domain of afadin, a protein that connects Nectin 3 to the actin cytoskeleton. This interaction is vital for maintaining the structural integrity of adherens junctions, where Nectin 3α co-localizes with Nectin 2, facilitating cell-cell adhesion and signaling. The ability of Nectin 3 to mediate these interactions underscores its importance in both normal cellular functions and in the pathology of diseases, including its role as a receptor for α herpesviruses, making Nectin 3 a significant target for research and therapeutic applications.

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Nectin 3 Antibody (N3.81.6) References:

1. Tage4/Nectin-like molecule-5 heterophilically trans-interacts with cell adhesion molecule Nectin-3 and enhances cell migration.  |  Ikeda, W., et al. 2003. J Biol Chem. 278: 28167-72. PMID: 12740392
2. Recruitment of nectin-3 to cell-cell junctions through trans-heterophilic interaction with CD155, a vitronectin and poliovirus receptor that localizes to alpha(v)beta3 integrin-containing membrane microdomains.  |  Mueller, S. and Wimmer, E. 2003. J Biol Chem. 278: 31251-60. PMID: 12759359
3. Secreted Frizzled-related protein 1 (sFRP1) regulates spermatid adhesion in the testis via dephosphorylation of focal adhesion kinase and the nectin-3 adhesion protein complex.  |  Wong, EW., et al. 2013. FASEB J. 27: 464-77. PMID: 23073828
4. Tauopathy differentially affects cell adhesion molecules in mouse brain: early down-regulation of nectin-3 in stratum lacunosum moleculare.  |  Maurin, H., et al. 2013. PLoS One. 8: e63589. PMID: 23704923
5. Nectin-3 (CD113) interacts with Nectin-2 (CD112) to promote lymphocyte transendothelial migration.  |  Devilard, E., et al. 2013. PLoS One. 8: e77424. PMID: 24116228
6. The expression of the Nectin complex in human breast cancer and the role of Nectin-3 in the control of tight junctions during metastasis.  |  Martin, TA., et al. 2013. PLoS One. 8: e82696. PMID: 24386110
7. Nectin expression in pancreatic adenocarcinoma: nectin-3 is associated with a poor prognosis.  |  Izumi, H., et al. 2015. Surg Today. 45: 487-94. PMID: 25690753
8. A new method for detection of tumor driver-dependent changes of protein sialylation in a colon cancer cell line reveals nectin-3 as TGFBR2 target.  |  Lee, J., et al. 2015. Protein Sci. 24: 1686-94. PMID: 26177744
9. Loss of nectin-3 expression as a marker of tumor aggressiveness in pancreatic neuroendocrine tumor.  |  Hirabayashi, K., et al. 2020. Pathol Int. 70: 84-91. PMID: 31855317
10. Precise levels of nectin-3 are required for proper synapse formation in postnatal visual cortex.  |  Tomorsky, J., et al. 2020. Neural Dev. 15: 13. PMID: 33160402

The preferred Nectin 3 antibody is Nectin 3 Antibody (H-11): sc-271611.