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Nectin 3 Antibody (H-11): sc-271611

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Datasheets
  • Nectin 3 Antibody (H-11) is a mouse monoclonal IgG1 κ Nectin 3 antibody, cited in 1 publications, provided at 200 µg/ml
  • raised against amino acids 301-545 mapping near the C-terminus of Nectin 3 of human origin
  • Nectin 3 Antibody (H-11) is recommended for detection of Nectin 3 of mouse, rat and human origin by WB, IP, IF and ELISA
  • Anti-Nectin 3 Antibody (H-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Nectin 3 Antibody (H-11) for WB applications. These reagents are now offered in bundles with Nectin 3 Antibody (H-11) (see ordering information below).

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Nectin 3 Antibody (H-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects Nectin 3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Nectin 3 antibody (H-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Nectin 3, also known as poliovirus receptor-related protein 3 (PRR3), plays a crucial role in cell adhesion and is a member of the immunoglobulin superfamily. Nectin 3 is primarily located in the cell membrane, where Nectin 3 facilitates homophilic interactions between adjacent cells, which is essential for maintaining tissue architecture and integrity. Nectin 3′s presence at cadherin-based adherens junctions allows for stable cell-cell contacts formation, vital for processes such as tissue development, immune response, and epithelial barrier maintenance. Nectin 3 interacts with the actin cytoskeleton through a C-terminal conserved motif, linking to F-actin-binding protein afadin, thereby influencing cellular signaling pathways and cytoskeletal dynamics. Three splicing variants—Nectin 3α, 3β, and 3γ—exhibit distinct functional properties, with Nectin 3α and 3β being particularly important for interactions with afadin and other cellular components. This multifaceted role underscores Nectin 3′s significance in both normal physiological processes and potential pathological conditions, making Nectin 3 (H-11) monoclonal antibody an invaluable tool for researchers studying cell adhesion mechanisms and related diseases.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Nectin 3 Antibody (H-11) References:

  1. Similar and differential behaviour between the nectin-afadin-ponsin and cadherin-catenin systems during the formation and disruption of the polarized junctional alignment in epithelial cells.  |  Asakura, T., et al. 1999. Genes Cells. 4: 573-81. PMID: 10583506
  2. Nectin-3, a new member of immunoglobulin-like cell adhesion molecules that shows homophilic and heterophilic cell-cell adhesion activities.  |  Satoh-Horikawa, K., et al. 2000. J Biol Chem. 275: 10291-9. PMID: 10744716
  3. Cell-cell adhesion-mediated tyrosine phosphorylation of nectin-2delta, an immunoglobulin-like cell adhesion molecule at adherens junctions.  |  Kikyo, M., et al. 2000. Oncogene. 19: 4022-8. PMID: 10962558
  4. Human nectin3/PRR3: a novel member of the PVR/PRR/nectin family that interacts with afadin.  |  Reymond, N., et al. 2000. Gene. 255: 347-55. PMID: 11024295
  5. Expression of nectin-1, nectin-3, N-cadherin, and NCAM in spinal motoneurons after sciatic nerve transection.  |  Zelano, J., et al. 2006. Exp Neurol. 201: 461-9. PMID: 16777094
  6. Nectin-3 (CD113) interacts with Nectin-2 (CD112) to promote lymphocyte transendothelial migration.  |  Devilard, E., et al. 2013. PLoS One. 8: e77424. PMID: 24116228
  7. Nectin-3 Is Increased in the Cell Junctions of the Uterine Epithelium at Implantation.  |  Poon, CE., et al. 2016. Reprod Sci. 23: 1580-1592. PMID: 27217376
  8. Nectin-3 modulates the structural plasticity of dentate granule cells and long-term memory.  |  Wang, XX., et al. 2017. Transl Psychiatry. 7: e1228. PMID: 28872640
  9. Nectin-3 is a new biomarker that mediates the upregulation of MMP2 and MMP9 in ovarian cancer cells.  |  Xu, F., et al. 2019. Biomed Pharmacother. 110: 139-144. PMID: 30469078
  10. Loss of nectin-3 expression as a marker of tumor aggressiveness in pancreatic neuroendocrine tumor.  |  Hirabayashi, K., et al. 2020. Pathol Int. 70: 84-91. PMID: 31855317
  11. Evidence that Nectin-3 is the soybean agglutinin binding protein on rat corneal endothelium cell surfaces.  |  Gordon, SR., et al. 2022. Exp Eye Res. 223: 109212. PMID: 35964705
  12. Complementary DNA characterization and chromosomal localization of a human gene related to the poliovirus receptor-encoding gene.  |  Lopez, M., et al. 1995. Gene. 155: 261-5. PMID: 7721102

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Nectin 3 Antibody (H-11)

sc-271611
200 µg/ml
$316.00

Nectin 3 Antibody (H-11): m-IgG Fc BP-HRP Bundle

sc-529222
200 µg Ab; 10 µg BP
$354.00

Nectin 3 Antibody (H-11): m-IgGκ BP-HRP Bundle

sc-521997
200 µg Ab, 40 µg BP
$354.00

Nectin 3 Antibody (H-11): m-IgG1 BP-HRP Bundle

sc-543397
200 µg Ab; 20 µg BP
$354.00

Nectin 3 Antibody (H-11) AC

sc-271611 AC
500 µg/ml, 25% agarose
$416.00

Nectin 3 Antibody (H-11) HRP

sc-271611 HRP
200 µg/ml
$316.00

Nectin 3 Antibody (H-11) FITC

sc-271611 FITC
200 µg/ml
$330.00

Nectin 3 Antibody (H-11) PE

sc-271611 PE
200 µg/ml
$343.00

Nectin 3 Antibody (H-11) Alexa Fluor® 488

sc-271611 AF488
200 µg/ml
$357.00

Nectin 3 Antibody (H-11) Alexa Fluor® 546

sc-271611 AF546
200 µg/ml
$357.00

Nectin 3 Antibody (H-11) Alexa Fluor® 594

sc-271611 AF594
200 µg/ml
$357.00

Nectin 3 Antibody (H-11) Alexa Fluor® 647

sc-271611 AF647
200 µg/ml
$357.00

Nectin 3 Antibody (H-11) Alexa Fluor® 680

sc-271611 AF680
200 µg/ml
$357.00

Nectin 3 Antibody (H-11) Alexa Fluor® 790

sc-271611 AF790
200 µg/ml
$357.00

Are there any issues with using sc-271611: Nectin 3 (H-11) mouse monoclonal primary antibody on mouse samples?

Asked by: Professor Griffin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-271611 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 5 out of 5 by from Great antibodyWorks very well in human neural stem cells. Visible band at 100 kDa.
Date published: 2017-12-14
Rated 5 out of 5 by from Nectin-3 antibodyExcelent antibody for western blot. Detected Nectin-3 at expected size in 15 ug total proteine lysate, wet transfer one hour at 450 mAmps on PVDF membrane, overnight blocking with 5% nonfat dry milk in PBS, one hour incubation at RT with 1:500 anti Nectin-3 in 5% PBS with 0,2%Tween followed by one hour incubation with 1:10.000 anti mouse (sc-358914) HRP conjugated antibody and detection with ECL prime substrate followed by short film exposure (under 1 minute) and automatic processing.
Date published: 2017-09-25
Rated 5 out of 5 by from very satisfiedWB investigate the expression of Nectin 3 in breast cancer tissues ,very satisfied with strong signal at 83kda,use the dilution 1:800.
Date published: 2017-09-03
Rated 4 out of 5 by from good quality antibodyNectin 3 antibody is a good antibody for detection of Nectin 3 by WB (1:800, 5% BSA,TBST),we got specific band, but the IF result have some background signal,the condition should be optimized.
Date published: 2017-07-29
Rated 5 out of 5 by from gave strong signalThis antibody gave strong signal in Hepg2 cell lysates ,I got target band. Dilution was 1:1000 and there were no visibility of background signals.
Date published: 2017-07-08
Rated 5 out of 5 by from Produced nice Western blot data of NectinProduced nice Western blot data of Nectin 3 expression in 3T3-L1 whole cell lysate. -SCBT QC
Date published: 2014-06-26
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Nectin 3 Antibody (H-11) is rated 4.8 out of 5 by 6.
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