Date published: 2026-2-10

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NDUFA5 Antibody (E-5): sc-393798

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Datasheets
  • NDUFA5 Antibody (E-5) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
  • raised against amino acids 1-116 representing full length NDUFA5 of human origin
  • recommended for detection of NDUFA5 of human origin by WB, IP, IF and ELISA
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for NDUFA5 Antibody (E-5) for WB applications. These reagents are now offered in bundles with NDUFA5 Antibody (E-5) (see ordering information below).
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    NDUFA5 Antibody (E-5) is a mouse monoclonal IgG1 antibody that detects NDUFA5 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NDUFA5, also known as Complex I-13kD-B, is a crucial component of the mitochondrial electron transport chain, specifically as one of the 45 subunits of complex I, which plays a vital role in oxidative phosphorylation. NDUFA5 facilitates the transfer of electrons from NADH to ubiquinone, ultimately contributing to ATP synthesis. Deficiencies in complex I can lead to severe metabolic disorders, affecting multiple organ systems, including the heart, liver, and kidneys. NDUFA5 is a 116 amino acid protein widely expressed, with highest levels found in the heart, skeletal muscle, and brain, highlighting NDUFA5′s importance in energy-demanding tissues. Understanding NDUFA5′s role and interactions within the electron transport chain is vital for elucidating mechanisms underlying mitochondrial dysfunction and associated pathologies.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    NDUFA5 Antibody (E-5)

    sc-393798
    200 µg/ml
    $322.00

    NDUFA5 Antibody (E-5): m-IgG Fc BP-HRP Bundle

    sc-538311
    200 µg Ab; 10 µg BP
    $361.00

    NDUFA5 Antibody (E-5): m-IgGκ BP-HRP Bundle

    sc-535893
    200 µg Ab; 40 µg BP
    $361.00

    NDUFA5 Antibody (E-5): m-IgG1 BP-HRP Bundle

    sc-545634
    200 µg Ab; 20 µg BP
    $361.00

    How much sample do you recommend loading on the gel for Western blot detection using NDUFA5 (E-5): sc-393798?

    Asked by: jenniferc
    We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
    Answered by: Technical Support
    Date published: 2017-03-01
    • y_2026, m_1, d_23, h_8CST
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    Rated 5 out of 5 by from good antibody!strong signal and low background. need to use 4-20% gradient gel
    Date published: 2025-07-22
    Rated 5 out of 5 by from Satisfactory Western Blot data of NDUFA5Satisfactory Western Blot data of NDUFA5 expression in human heart tissue extract and A-673 whole cell lysate . -SCBT QC
    Date published: 2015-05-10
    Rated 5 out of 5 by from Great Western Blot data of NDUFA5 expressionGreat Western Blot data of NDUFA5 expression in Hep G2 and HeLa whole cell lysates and human kidney tissue extract . -SCBT QC
    Date published: 2014-02-04
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    NDUFA5 Antibody (E-5) is rated 5.0 out of 5 by 3.
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