NDUFA5 Antibody (A-3): sc-393273

NDUFA5 Antibody (A-3) is a mouse monoclonal IgG2a kappa light chain antibody that detects NDUFA5 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NDUFA5 (A-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NDUFA5, also known as NADH-ubiquinone oxidoreductase a subunit 5 or Complex I-13kD-B, is a crucial component of the mitochondrial oxidative phosphorylation pathway, comprising one of the 45 subunits of complex I, the first enzyme complex in the electron transport chain. The proper functioning of NDUFA5 is vital for ATP production, as NDUFA5 facilitates the transfer of electrons from NADH to ubiquinone, a key step in cellular respiration. Deficiencies in complex I, where NDUFA5 plays a significant role, are linked to a range of serious health issues, including cardiac, hepatic, and renal disorders, making the study of NDUFA5 essential for understanding mitochondrial dysfunction. NDUFA5 is a 116 amino acid protein that is widely expressed, with the highest levels found in the heart, skeletal muscle, and brain, highlighting NDUFA5′s importance in energy metabolism across various tissues.

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NDUFA5 Antibody (A-3) References:

1. Genomic organization of the human complex I 13-kDa subunit gene NDUFA5.  |  Tensing, K., et al. 1999. Cytogenet Cell Genet. 84: 125-7. PMID: 10343126
2. Levels of human Fis1 at the mitochondrial outer membrane regulate mitochondrial morphology.  |  Stojanovski, D., et al. 2004. J Cell Sci. 117: 1201-10. PMID: 14996942
3. Localization of the human 75-kDal Fe-S protein of NADH-coenzyme Q reductase gene (NDUFS1) to 2q33----q34.  |  Duncan, AM., et al. 1992. Cytogenet Cell Genet. 60: 212-3. PMID: 1505218
4. Leigh syndrome associated with mitochondrial complex I deficiency due to a novel mutation in the NDUFS1 gene.  |  Martín, MA., et al. 2005. Arch Neurol. 62: 659-61. PMID: 15824269
5. A high-fat diet coordinately downregulates genes required for mitochondrial oxidative phosphorylation in skeletal muscle.  |  Sparks, LM., et al. 2005. Diabetes. 54: 1926-33. PMID: 15983191
6. Ultrasound evaluation of peritoneal catheter tunnel in catheter related infections in CAPD.  |  Karahan, OI., et al. 2005. Int Urol Nephrol. 37: 363-6. PMID: 16142572
7. Cell biological consequences of mitochondrial NADH: ubiquinone oxidoreductase deficiency.  |  Smeitink, JA., et al. 2004. Curr Neurovasc Res. 1: 29-40. PMID: 16181064
8. Identification of NADH dehydrogenase 1 alpha subcomplex 5 capable to transform murine fibroblasts and overexpressed in human cervical carcinoma cell lines.  |  Shimada, T., et al. 2006. Biochem Biophys Res Commun. 339: 852-7. PMID: 16329995
9. A possible role for iron-sulfur cluster N2 in proton translocation by the NADH: ubiquinone oxidoreductase (complex I).  |  Flemming, D., et al. 2005. J Mol Microbiol Biotechnol. 10: 208-22. PMID: 16645316
10. Topography and chemical reactivity of the active-inactive transition-sensitive SH-group in the mitochondrial NADH:ubiquinone oxidoreductase (Complex I).  |  Gostimskaya, IS., et al. 2006. Biochim Biophys Acta. 1757: 1155-61. PMID: 16777054
11. Determination of the cDNA sequence for the human mitochondrial 75-kDa Fe-S protein of NADH-coenzyme Q reductase.  |  Chow, W., et al. 1991. Eur J Biochem. 201: 547-50. PMID: 1935949

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NDUFA5 Antibody (A-3) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-NDUFA5 Antibody (A-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NDUFA5 Antibody (A-3) Specifications

Alternate Product Name: NDUFA5

Product Clone Name: A-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-116

Epitope Target: NDUFA5

Epitope Species: human

Product Reactivity/Detection: NDUFA5

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for NDUFA5 Antibody (A-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin