Ndfip1 Antibody (H-6): sc-515417

Ndfip1 Antibody (H-6) is a mouse monoclonal IgG2b kappa light chain antibody that detects Ndfip1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Ndfip1 antibody (H-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Nedd4 family interacting protein-1 (Ndfip1), also known as N4WBP5, is a highly conserved protein that plays a crucial role in cellular processes, particularly in protein degradation regulation through interaction with HECT-type E3 ubiquitin ligases. Ndfip1 features three transmembrane domains at the carboxy terminus and two proline-tyrosine (PY) motifs at the amino terminus, which are essential for function. Ndfip1 predominantly resides in the Golgi apparatus, influencing Golgi structure and function, thereby impacting protein trafficking and processing within cells. Ndfip1 shows strong expression in neurons surrounding injury sites, suggesting a potential neuroprotective role by facilitating ubiquitination processes that may promote cell survival. Ndfip1 expression increases upon T cell activation, indicating involvement in immune responses. Mice lacking Ndfip1 develop severe skin and lung inflammation due to Itch inactivation, another E3 ubiquitin ligase, leading to premature mortality, highlighting Ndfip1′s importance in maintaining cellular homeostasis and potential implications in neuroprotection and immune regulation.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Ndfip1 Antibody (H-6) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-Ndfip1 Antibody (H-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Ndfip1 Antibody (H-6) Specifications

Alternate Product Name: Ndfip1

Product Clone Name: H-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 85-110

Epitope Target: Ndfip1

Epitope Species: human

Product Reactivity/Detection: Ndfip1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Ndfip1 Antibody (H-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred Ndfip1 antibody is Ndfip1 Antibody (D-4): sc-398469.