NCB5OR Antibody (D-4): sc-390570

NCB5OR Antibody (D-4) is a mouse monoclonal IgG1 antibody that detects NCB5OR in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NCB5OR, also known as cytochrome b5 reductase 4, is a flavohemoprotein that plays a vital role in cellular metabolism by facilitating the reduction of various substrates, including cytochrome c and methemoglobin, which is crucial for maintaining redox balance within the cell. This protein is predominantly located in the endoplasmic reticulum, where NCB5OR colocalizes with calreticulin, highlighting NCB5OR′s importance in the endoplasmic reticulum stress response pathway. By mitigating oxidative stress and preventing the accumulation of reactive oxygen species, NCB5OR is essential for protecting pancreatic beta-cells, and NCB5OR deficiency can lead to insulin-deficient diabetes. The structural features of NCB5OR, including cytochrome b5 heme-binding domain and multiple binding motifs for flavin adenine dinucleotide and iron, further underscore NCB5OR′s functional significance in cellular processes. Anti-NCB5OR antibody (D-4) is an invaluable tool for researchers studying the implications of NCB5OR in metabolic disorders and oxidative stress responses.

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NCB5OR Antibody (D-4) References:

1. NCB5OR is a novel soluble NAD(P)H reductase localized in the endoplasmic reticulum.  |  Zhu, H., et al. 2004. J Biol Chem. 279: 30316-25. PMID: 15131110
2. Absence of a reductase, NCB5OR, causes insulin-deficient diabetes.  |  Xie, J., et al. 2004. Proc Natl Acad Sci U S A. 101: 10750-5. PMID: 15247412
3. Variation in NCB5OR: studies of relationships to type 2 diabetes, maturity-onset diabetes of the young, and gestational diabetes mellitus.  |  Andersen, G., et al. 2004. Diabetes. 53: 2992-7. PMID: 15504981
4. Study of the individual cytochrome b5 and cytochrome b5 reductase domains of Ncb5or reveals a unique heme pocket and a possible role of the CS domain.  |  Deng, B., et al. 2010. J Biol Chem. 285: 30181-91. PMID: 20630863
5. Ncb5or deficiency increases fatty acid catabolism and oxidative stress.  |  Xu, M., et al. 2011. J Biol Chem. 286: 11141-54. PMID: 21300801
6. Activation and detoxification metabolism of urban air pollutants 2-nitrobenzanthrone and carcinogenic 3-nitrobenzanthrone by rat and mouse hepatic microsomes.  |  Stiborova, M., et al. 2012. Neuro Endocrinol Lett. 33 Suppl 3: 8-15. PMID: 23353838
7. Natural mutations lead to enhanced proteasomal degradation of human Ncb5or, a novel flavoheme reductase.  |  Kálmán, FS., et al. 2013. Biochimie. 95: 1403-10. PMID: 23523930
8. Cytosolic localization of NADH cytochrome b₅ oxidoreductase (Ncb5or).  |  Zámbó, V., et al. 2016. FEBS Lett. 590: 661-71. PMID: 26878259
9. Crystal structures of the naturally fused CS and cytochrome b5 reductase (b5R) domains of Ncb5or reveal an expanded CS fold, extensive CS-b5R interactions and productive binding of the NAD(P)+ nicotinamide ring.  |  Benson, DR., et al. 2019. Acta Crystallogr D Struct Biol. 75: 628-638. PMID: 31282472
10. The N-terminal intrinsically disordered region of Ncb5or docks with the cytochrome b5 core to form a helical motif that is of ancient origin.  |  Benson, DR., et al. 2024. Proteins. 92: 554-566. PMID: 38041394

The preferred NCB5OR antibody is NCB5OR Antibody (D-2): sc-390569.