NCB5OR Antibody (D-2): sc-390569
- NCB5OR Antibody (D-2) is a mouse monoclonal IgG2a κ NCB5OR antibody provided at 200 µg/ml
- raised against amino acids 331-521 mapping at the C-terminus of NCB5OR of human origin
- NCB5OR Antibody (D-2) is recommended for detection of NCB5OR of human origin by WB, IP, IF and ELISA
- Anti-NCB5OR Antibody (D-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for NCB5OR Antibody (D-2) for WB applications. These reagents are now offered in bundles with NCB5OR Antibody (D-2) (see ordering information below).
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NCB5OR Antibody (D-2) is a mouse monoclonal IgG2a kappa light chain antibody that detects NCB5OR of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NCB5OR (D-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NCB5OR, also known as cytochrome b5 reductase 4 (CYB5R4), is a flavohemoprotein that plays a vital role in cellular metabolism and redox reactions. NCB5OR is primarily located in the endoplasmic reticulum, where NCB5OR colocalizes with calreticulin, highlighting NCB5OR′s importance in the stress response of the endoplasmic reticulum. NCB5OR contains a cytochrome b5 heme-binding domain, one CS domain, two FAD binding motifs, and two iron binding motifs, which are crucial for enzymatic activity. NCB5OR is involved in reducing various substrates, including cytochrome c, methemoglobin, ferricyanide, and molecular oxygen, thereby contributing to cellular protection against oxidative stress. NCB5OR is essential for safeguarding pancreatic beta-cells from oxidative damage, and NCB5OR absence can lead to insulin-deficient diabetes, underscoring NCB5OR′s significance in metabolic health.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
NCB5OR Antibody (D-2) References:
- NCB5OR is a novel soluble NAD(P)H reductase localized in the endoplasmic reticulum. | Zhu, H., et al. 2004. J Biol Chem. 279: 30316-25. PMID: 15131110
- Absence of a reductase, NCB5OR, causes insulin-deficient diabetes. | Xie, J., et al. 2004. Proc Natl Acad Sci U S A. 101: 10750-5. PMID: 15247412
- NADH cytochrome b5 reductase and cytochrome b5 catalyze the microsomal reduction of xenobiotic hydroxylamines and amidoximes in humans. | Kurian, JR., et al. 2004. J Pharmacol Exp Ther. 311: 1171-8. PMID: 15302896
- Variation in NCB5OR: studies of relationships to type 2 diabetes, maturity-onset diabetes of the young, and gestational diabetes mellitus. | Andersen, G., et al. 2004. Diabetes. 53: 2992-7. PMID: 15504981
- Promoter characterization and transcriptional regulation of Ncb5or, a novel reductase necessary for pancreatic beta-cell maintenance. | Larade, K. and Bunn, HF. 2006. Biochim Biophys Acta. 1759: 257-62. PMID: 16814408
- The reductase NCB5OR is responsive to the redox status in beta-cells and is not involved in the ER stress response. | Larade, K., et al. 2007. Biochem J. 404: 467-76. PMID: 17343567
- Discovery and characterization of a cytochrome b5 variant in humans with impaired hydroxylamine reduction capacity. | Kurian, JR., et al. 2007. Pharmacogenet Genomics. 17: 597-603. PMID: 17622936
- Ncb5or deficiency increases fatty acid catabolism and oxidative stress. | Xu, M., et al. 2011. J Biol Chem. 286: 11141-54. PMID: 21300801
- Natural mutations lead to enhanced proteasomal degradation of human Ncb5or, a novel flavoheme reductase. | Kálmán, FS., et al. 2013. Biochimie. 95: 1403-10. PMID: 23523930
- Cytosolic localization of NADH cytochrome b₅ oxidoreductase (Ncb5or). | Zámbó, V., et al. 2016. FEBS Lett. 590: 661-71. PMID: 26878259
- The N-terminal intrinsically disordered region of Ncb5or docks with the cytochrome b5 core to form a helical motif that is of ancient origin. | Benson, DR., et al. 2024. Proteins. 92: 554-566. PMID: 38041394
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NCB5OR Antibody (D-2) | sc-390569 | 200 µg/ml | $316.00 | |||
NCB5OR Antibody (D-2): m-IgG Fc BP-HRP Bundle | sc-526172 | 200 µg Ab; 10 µg BP | $354.00 | |||
NCB5OR Antibody (D-2): m-IgG2a BP-HRP Bundle | sc-546521 | 200 µg Ab; 10 µg BP | $354.00 | |||
NCB5OR Antibody (D-2) AC | sc-390569 AC | 500 µg/ml, 25% agarose | $416.00 | |||
NCB5OR Antibody (D-2) HRP | sc-390569 HRP | 200 µg/ml | $316.00 | |||
NCB5OR Antibody (D-2) FITC | sc-390569 FITC | 200 µg/ml | $330.00 | |||
NCB5OR Antibody (D-2) PE | sc-390569 PE | 200 µg/ml | $343.00 | |||
NCB5OR Antibody (D-2) Alexa Fluor® 488 | sc-390569 AF488 | 200 µg/ml | $357.00 | |||
NCB5OR Antibody (D-2) Alexa Fluor® 546 | sc-390569 AF546 | 200 µg/ml | $357.00 | |||
NCB5OR Antibody (D-2) Alexa Fluor® 594 | sc-390569 AF594 | 200 µg/ml | $357.00 | |||
NCB5OR Antibody (D-2) Alexa Fluor® 647 | sc-390569 AF647 | 200 µg/ml | $357.00 | |||
NCB5OR Antibody (D-2) Alexa Fluor® 680 | sc-390569 AF680 | 200 µg/ml | $357.00 | |||
NCB5OR Antibody (D-2) Alexa Fluor® 790 | sc-390569 AF790 | 200 µg/ml | $357.00 |