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NCAM/CD56 Antibody (D-10): sc-374289

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Datasheets
  • NCAM/CD56 Antibody (D-10) is a mouse monoclonal IgG2a κ provided at 200 µg/ml
  • raised against amino acids 1-300 mapping at the N-terminus of NCAM of human origin
  • recommended for detection of NCAM of human origin by WB, IP, IF, IHC(P) and ELISA
  • See NCAM/CD56 (123C3): sc-7326 for NCAM/CD56 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for NCAM/CD56 Antibody (D-10) for WB and IHC(P) applications. These reagents are now offered in bundles with NCAM/CD56 Antibody (D-10) (see ordering information below).

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    NCAM Antibody (D-10) is a mouse monoclonal IgG2a kappa light chain antibody specifically designed to target the Neural Cell Adhesion Molecule (NCAM), also known as CD56. anti-NCAM antibody (D-10) is raised against amino acids 1-300 at the N-terminus of human NCAM, ensuring precise recognition and binding. NCAM (D-10) antibody is highly effective for detecting human NCAM in various applications, including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). NCAM is a vital cell surface glycoprotein involved in mediating cell-to-cell interactions, playing a crucial role in embryogenesis, neural development, and synaptic plasticity. NCAM facilitates important biological processes such as neurite outgrowth and neuronal differentiation by interacting with proteins like fibroblast growth factor receptors (FGFRs), integrins, and intracellular signaling molecules such as Fyn and focal adhesion kinase (FAK). Beyond its essential functions in normal physiology, NCAM is a significant marker in various human tumors, including neuroblastomas, rhabdomyosarcomas, Wilms′ tumor, and Ewing′s sarcoma. NCAM (D-10) monoclonal antibody is an invaluable tool for researchers studying the molecular mechanisms underlying neural development and tumor biology, providing reliable detection and analysis of NCAM expression. anti-NCAM antibody (D-10) supports advancements in both developmental biology and cancer research by facilitating the investigation of NCAM interactions and signaling pathways.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    NCAM/CD56 Antibody (D-10)

    sc-374289
    200 µg/ml
    $322.00

    NCAM/CD56 Antibody (D-10): m-IgG Fc BP-HRP Bundle

    sc-537949
    200 µg Ab; 10 µg BP
    $361.00

    NCAM/CD56 Antibody (D-10): m-IgGκ BP-HRP Bundle

    sc-535437
    200 µg Ab; 40 µg BP
    $361.00

    NCAM/CD56 Antibody (D-10): m-IgG2a BP-HRP Bundle

    sc-548157
    200 µg Ab; 10 µg BP
    $361.00

    Can NCAM (D-10): sc-374289 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

    Asked by: jenniferc
    Thank you for your question. Yes, NCAM (D-10): sc-374289 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
    Answered by: Technical Support
    Date published: 2017-02-27
    • y_2026, m_1, d_1, h_8CST
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    Rated 5 out of 5 by from Produced superb immunoperoxidase membraneProduced superb immunoperoxidase membrane and cytoplasmic staining in formalin fixed, paraffin-embedded human adrenal gland tissue. -SCBT QC
    Date published: 2015-04-05
    Rated 5 out of 5 by from Satisfactory Western Blot data of NCAM expressionSatisfactory Western Blot data of NCAM expression in non-transfected and human NCAM transfected 293T whole cell lysates. -SCBT QC
    Date published: 2013-02-03
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    NCAM/CD56 Antibody (D-10) is rated 5.0 out of 5 by 2.
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