Myosin VI Antibody (G-6): sc-398609

Myosin VI Antibody (G-6) is a mouse monoclonal IgG1 antibody that detects Myosin VI in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Myosin VI is a unique molecular motor that plays a critical role in intracellular vesicle and organelle transport, distinguished by binding to the pointed end of actin filaments, a feature not shared by other myosins. Myosin VI is essential for endocytosis, associating with clathrin-coated vesicles and interacting with proteins like disabled 2, highlighting importance in cellular trafficking processes. Myosin VI is predominantly expressed in the sensory hair cells of the cochlea, where maintaining the structural integrity of stereocilia bundles is crucial; defects can lead to autosomal dominant nonsyndromic sensorineural deafness in humans. The human Myosin VI gene is located in the centromeric region of chromosome 6, showing syntenic homology with mouse chromosome 9, where the Snell′s Waltzer mutation is found. This mutation results in significant auditory and behavioral impairments, including hyperactivity and lack of responsiveness to sound, due to stereocilia disorganization in the inner ear. Anti-Myosin VI antibody (G-6) serves as an invaluable tool for researchers studying molecular mechanisms underlying hearing loss and cellular dynamics of vesicle transport.

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Myosin VI Antibody (G-6) References:

1. Myosin VI and its cargo adaptors - linking endocytosis and autophagy.  |  Tumbarello, DA., et al. 2013. J Cell Sci. 126: 2561-70. PMID: 23781020
2. Myosin VI facilitates connexin 43 gap junction accretion.  |  Waxse, BJ., et al. 2017. J Cell Sci. 130: 827-840. PMID: 28096472
3. Myosin VI-Dependent Actin Cages Encapsulate Parkin-Positive Damaged Mitochondria.  |  Kruppa, AJ., et al. 2018. Dev Cell. 44: 484-499.e6. PMID: 29398621
4. Myosin VI Drives Clathrin-Mediated AMPA Receptor Endocytosis to Facilitate Cerebellar Long-Term Depression.  |  Wagner, W., et al. 2019. Cell Rep. 28: 11-20.e9. PMID: 31269433
5. Dynamic multimerization of Dab2-Myosin VI complexes regulates cargo processivity while minimizing cortical actin reorganization.  |  Rai, A., et al. 2021. J Biol Chem. 296: 100232. PMID: 33372034
6. Myosin VI regulates ciliogenesis by promoting the turnover of the centrosomal/satellite protein OFD1.  |  Magistrati, E., et al. 2022. EMBO Rep. 23: e54160. PMID: 34957672
7. Multimodal regulation of myosin VI ensemble transport by cargo adaptor protein GIPC.  |  Rai, A., et al. 2022. J Biol Chem. 298: 101688. PMID: 35143838
8. Myosin VI regulates the spatial organisation of mammalian transcription initiation.  |  Hari-Gupta, Y., et al. 2022. Nat Commun. 13: 1346. PMID: 35292632
9. Binding partners regulate unfolding of myosin VI to activate the molecular motor.  |  Dos Santos, Á., et al. 2022. Biochem J. 479: 1409-1428. PMID: 35722941
10. Unconventional myosin VI in the heart: Involvement in cardiac dysfunction progressing with age.  |  Karatsai, O., et al. 2023. Biochim Biophys Acta Mol Basis Dis. 1869: 166748. PMID: 37169038

The preferred Myosin VI antibody is Myosin VI Antibody (A-9): sc-393558.