Myosin VI Antibody (A-9): sc-393558

Myosin VI Antibody (A-9) is a mouse monoclonal IgG3 kappa light chain antibody that detects Myosin VI (MYO6) of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-Myosin VI antibody (A-9) is available as a non-conjugated format. Myosin VI (MYO6) plays a crucial role as a molecular motor in intracellular vesicle and organelle transport, uniquely binding to the pointed end of actin filaments, which is essential for cellular processes. MYO6 has only one light chain in its lever-arm domain, resulting in highly irregular stepping patterns and diverse step sizes compared to other myosins. Myosin VI (MYO6) associates with Clathrin-coated vesicles and disabled 2, highlighting significant involvement in endocytosis. Mouse MYO6 is predominantly expressed in the sensory hair cells of the cochlea, where MYO6 is vital for auditory function. Human MYO6 is mapped to the centromeric region of chromosome 6, which shares syntenic homology with the corresponding region on mouse chromosome 9, where the Snell′s Waltzer mutation is located. This mutation leads to behavioral effects such as lack of responsiveness to sound, hyperactivity, head tossing, and circling, attributed to the disorganization and fusion of stereocilia bundles in the inner ear. Defects in MYO6 are linked to autosomal dominant nonsyndromic sensorineural deafness in humans, underscoring MYO6′s critical role in hearing. Human MYO6 is expressed in the fetal cochlea and brain, as well as in the adult brain, emphasizing MYO6′s importance in both developmental and adult physiology.

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Myosin VI Antibody (A-9) References:

1. Myosin VI is an actin-based motor that moves backwards.  |  Wells, AL., et al. 1999. Nature. 401: 505-8. PMID: 10519557
2. Role of myosin VI in the differentiation of cochlear hair cells.  |  Self, T., et al. 1999. Dev Biol. 214: 331-41. PMID: 10525338
3. Genomic structure of the human unconventional myosin VI gene.  |  Ahituv, N., et al. 2000. Gene. 261: 269-75. PMID: 11167014
4. Myosin VI isoform localized to clathrin-coated vesicles with a role in clathrin-mediated endocytosis.  |  Buss, F., et al. 2001. EMBO J. 20: 3676-84. PMID: 11447109
5. MYO6, the human homologue of the gene responsible for deafness in Snell's waltzer mice, is mutated in autosomal dominant nonsyndromic hearing loss.  |  Melchionda, S., et al. 2001. Am J Hum Genet. 69: 635-40. PMID: 11468689
6. Myosin VI is a processive motor with a large step size.  |  Rock, RS., et al. 2001. Proc Natl Acad Sci U S A. 98: 13655-9. PMID: 11707568
7. Myosin VI binds to and localises with Dab2, potentially linking receptor-mediated endocytosis and the actin cytoskeleton.  |  Morris, SM., et al. 2002. Traffic. 3: 331-41. PMID: 11967127
8. Diverse functions of myosin VI in spermiogenesis.  |  Zakrzewski, P., et al. 2021. Histochem Cell Biol. 155: 323-340. PMID: 33386429
9. Nuclear myosin VI maintains replication fork stability.  |  Shi, J., et al. 2023. Nat Commun. 14: 3787. PMID: 37355687
10. Autoinhibition and activation of myosin VI revealed by its cryo-EM structure.  |  Niu, F., et al. 2024. Nat Commun. 15: 1187. PMID: 38331992
11. Myosin VI drives arrestin-independent internalization and signaling of GPCRs.  |  Patel, NM., et al. 2024. Nat Commun. 15: 10636. PMID: 39638791
12. Characterization of unconventional MYO6, the human homologue of the gene responsible for deafness in Snell's waltzer mice.  |  Avraham, KB., et al. 1997. Hum Mol Genet. 6: 1225-31. PMID: 9259267

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Myosin VI Antibody (A-9) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-Myosin VI Antibody (A-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Myosin VI Antibody (A-9) Specifications

Alternate Product Name: Myosin VI

Product Clone Name: A-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 1206-1237

Epitope Target: Myosin VI

Epitope Species: human

Product Reactivity/Detection: Myosin VI

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for Myosin VI Antibody (A-9): P: Blocking Peptide