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MyoD Antibody (E-1): sc-377186

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Datasheets
  • MyoD Antibody (E-1) is a mouse monoclonal IgG1 κ, cited in 11 publications, provided at 200 µg/ml
  • raised against amino acids 1-318 of MyoD of mouse origin
  • recommended for detection of MyoD of mouse and rat origin by WB, IP, IF and ELISA
  • TransCruz reagent for ChIP application (sc-377186 X, 200 µg/0.1 ml)
  • See MyoD (G-1): sc-377460 for MyoD antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MyoD Antibody (E-1) for WB applications. These reagents are now offered in bundles with MyoD Antibody (E-1) (see ordering information below).

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MyoD Antibody (E-1) is a mouse monoclonal IgG1 kappa light chain antibody designed to detect Myogenic Differentiation 1 (MyoD), also known as MRF1, a pivotal transcription factor within the Myogenic Regulatory Factors (MRFs) family that includes myogenin, Myf5, and MRF4. Anti-MyoD antibody (E-1) is raised against amino acids 1-318 of mouse MyoD, targeting a region that encompasses the basic helix-loop-helix (bHLH) domains, which are essential for dimerization with E proteins and binding to E-box sequences in DNA. The structural configuration of MyoD, particularly its bHLH domains, is critical for its role in regulating the transcription of muscle-specific genes, thereby driving muscle cell differentiation and development. Located in the nucleus, MyoD facilitates the activation of genes necessary for muscle regeneration by interacting with E proteins such as E2A, IF2, and HEB. MyoD monoclonal antibody (E-1) is recommended for use in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA) applications in both mouse and rat species. Available in both conjugated and non-conjugated forms, anti-MyoD antibody (E-1) offers versatility for various experimental protocols, making MyoD monoclonal antibody (E-1) a valuable tool for researchers exploring the molecular mechanisms of myogenesis, muscle biology, and related fields.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

MyoD Antibody (E-1)

sc-377186
200 µg/ml
$316.00

MyoD Antibody (E-1): m-IgG Fc BP-HRP Bundle

sc-538025
200 µg Ab; 10 µg BP
$354.00

MyoD Antibody (E-1): m-IgGκ BP-HRP Bundle

sc-535546
200 µg Ab; 40 µg BP
$354.00

MyoD Antibody (E-1): m-IgG1 BP-HRP Bundle

sc-545491
200 µg Ab; 20 µg BP
$354.00

MyoD Antibody (E-1) X

sc-377186 X
200 µg/0.1 ml
$316.00

How much sample do you recommend loading on the gel for Western blot detection using MyoD (E-1): sc-377186?

Asked by: AbPolly
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-02-24
  • y_2025, m_10, d_14, h_6CST
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Rated 5 out of 5 by from Excellent!This antibody works great in western blot analysis of MyoD expression in C2C12 whole cell lysate and Sol8 nuclear extract.
Date published: 2017-01-26
Rated 5 out of 5 by from Strong staining in IHC in mouse muscle tissuesStrong staining in IHC in mouse muscle tissues. -SCBT Publication Review
Date published: 2015-02-23
Rated 5 out of 5 by from Good specificity in WB in C2C12 whole cellGood specificity in WB in C2C12 whole cell lysate and Sol8 nuclear extract. -SCBT QC
Date published: 2015-01-11
Rated 5 out of 5 by from Good publishable data in WB in mice myoblastGood publishable data in WB in mice myoblast. -SCBT Publication Review
Date published: 2014-07-22
  • y_2025, m_10, d_14, h_6
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MyoD Antibody (E-1) is rated 5.0 out of 5 by 4.
  • y_2025, m_10, d_14, h_6
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  • cp_1, bvpage1
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