MyoD Antibody (G-1): sc-377460

MyoD Antibody (G-1) is a mouse monoclonal IgG2b kappa light chain antibody specifically designed to detect Myogenic Differentiation 1 (MyoD), also known as MYOD1, a crucial transcription factor within the Myogenic Regulatory Factors (MRFs) family that includes myogenin, Myf5, and MRF4 (herculin). anti-MyoD antibody (G-1) is raised against amino acids 1-318 of mouse MyoD, targeting a region that encompasses both the basic helix-loop-helix (bHLH) domains and other critical regions essential for MyoD′s function. MyoD plays a vital role in muscle cell differentiation by binding to E-box sequences (CANNTG) in DNA, thereby regulating the transcription of genes necessary for muscle development and regeneration. Post-translational modifications of MyoD, such as phosphorylation, acetylation, and ubiquitination, are essential for modulating stability, activity, and interactions with other proteins, making the detection of these modifications important for understanding the regulation of myogenesis. MyoD (G-1) monoclonal antibody is validated for use in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA) applications across multiple species, including mouse, rat, and human, without cross-reacting with other members of the MyoD family. MyoD (G-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for diverse experimental protocols.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

MyoD Antibody (G-1) References:

1. Crosstalk between cell cycle regulators and the myogenic factor MyoD in skeletal myoblasts.  |  Kitzmann, M. and Fernandez, A. 2001. Cell Mol Life Sci. 58: 571-9. PMID: 11361092
2. [New insight into MyoD regulation: involvement in rhabdomyosarcoma pathway?].  |  Tintignac, LA., et al. 2001. Bull Cancer. 88: 545-8. PMID: 11459700
3. MyoD synergizes with the E-protein HEB beta to induce myogenic differentiation.  |  Parker, MH., et al. 2006. Mol Cell Biol. 26: 5771-83. PMID: 16847330
4. Role of MyoD in denervated, disused, and exercised muscle.  |  Legerlotz, K. and Smith, HK. 2008. Muscle Nerve. 38: 1087-100. PMID: 18642380
5. MyoD is a tumor suppressor gene in medulloblastoma.  |  Dey, J., et al. 2013. Cancer Res. 73: 6828-37. PMID: 24092238
6. The histone demethylase KDM4B interacts with MyoD to regulate myogenic differentiation in C2C12 myoblast cells.  |  Choi, JH., et al. 2015. Biochem Biophys Res Commun. 456: 872-8. PMID: 25534856
7. PRMT1 activates myogenin transcription via MyoD arginine methylation at R121.  |  Liu, Q., et al. 2019. Biochim Biophys Acta Gene Regul Mech. 1862: 194442. PMID: 31634638
8. FOXP1 Interacts with MyoD to Repress its Transcription and Myoblast Conversion.  |  Wright, WE., et al. 2021. J Cell Signal. 2: 9-26. PMID: 33554216
9. Generation of a MyoD knock-in reporter mouse line to study muscle stem cell dynamics and heterogeneity.  |  Fujita, R., et al. 2023. iScience. 26: 106592. PMID: 37250337
10. MyoD and c-fos expression.  |  Walsh, K. 1993. Nature. 365: 611-2. PMID: 8413625

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MyoD Antibody (G-1) has 144 citations in a variety of scientific publications.

Primary antibodies like Anti-MyoD Antibody (G-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MyoD Antibody (G-1) Specifications

Alternate Product Name: MyoD

Product Clone Name: G-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-318

Epitope Target: MyoD

Epitope Species: mouse

Product Reactivity/Detection: MyoD

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 144 citations in various scientific publications

Available Conjugates/Forms for MyoD Antibody (G-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent

The preferred MyoD antibody is MyoD Antibody (5.8A): sc-32758.