Date published: 2026-2-7

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Mx1 抗体 (E-5): sc-271024

3.5(4)
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说明书
  • Mx1 抗体 E-5 是小鼠单克隆 IgG2a κ,Mx1抗体, 在5篇文献中引用,规格为200 µg/ml
  • 针对氨基酸171-455的图谱,在Mx1的内部区域,human起源
  • Mx1 抗体 (E-5) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 Mx1
  • 抗Mx1抗体(E-5)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • m-IgG Fc BP-HRPm-IgGκ BP-HRP是Mx1 Antibody (E-5) 适用于 WB 应用。 的首选辅助检测试剂,这些试剂现与Mx1 Antibody (E-5) 打包提供(请参阅下面的订购信息)。
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関連項目

Mx1抗体(E-5)是一种小鼠单克隆IgG2a kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和酶联免疫吸附测定(ELISA)检测小鼠、大鼠和人类来源的Mx1蛋白。抗 -Mx1抗体(E-5)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。Mx1蛋白,也称为粘病毒抗性蛋白1,在病毒感染的免疫反应中起着至关重要的作用,特别是在核定位方面。核定位使Mx1能够在病毒复制位点直接发挥抗病毒作用,从而对H5N1流感病毒等高致病性病毒产生选择性抵抗力。在α干扰素和β干扰素的诱导下,Mx1会积聚在细胞核中,干扰病毒生命周期并抑制病毒复制,从而增强宿主的防御机制。Mx1与免疫信号通路中其他蛋白的相互作用进一步凸显了其在宿主抗病毒反应中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Mx1 抗体 (E-5) 参考文献:

  1. 小鼠 Mx2 基因的鉴定:干扰素诱导的野鼠基因 Mx2 蛋白表达可产生对水泡性口炎病毒的抵抗力。  |  Jin, HK., et al. 1999. J Virol. 73: 4925-30. PMID: 10233954
  2. Mx 蛋白的功能多样性:宿主抗感染主题的变异。  |  Lee, SH. and Vidal, SM. 2002. Genome Res. 12: 527-30. PMID: 11932237
  3. 小鼠 Mx2 蛋白能抑制水泡性口炎病毒,但不能抑制流感病毒。  |  Zürcher, T., et al. 1992. Virology. 187: 796-800. PMID: 1312277
  4. 小鼠对流感病毒抵抗力的遗传。  |  LINDENMANN, J. 1964. Proc Soc Exp Biol Med. 116: 506-9. PMID: 14193387
  5. Mx1 基因可保护小鼠免受 1918 年大流行流感病毒和高度致命的人类 H5N1 流感病毒的侵袭。  |  Tumpey, TM., et al. 2007. J Virol. 81: 10818-21. PMID: 17652381
  6. Mx1 基因保护小鼠免受高致命性人类 H5N1 流感病毒的侵袭。  |  Salomon, R., et al. 2007. Cell Cycle. 6: 2417-21. PMID: 17700072
  7. 鉴定第二个受干扰素调控的小鼠 Mx 基因  |  Staeheli, P. and Sutcliffe, JG. 1988. Mol Cell Biol. 8: 4524-8. PMID: 2460745
  8. 小鼠 Mx 基因的组织结构及其干扰素和病毒诱导启动子的特征。  |  Hug, H., et al. 1988. Mol Cell Biol. 8: 3065-79. PMID: 2974922
  9. Mx 蛋白:在用克隆的 Mx cDNA 转化的 3T3 细胞中进行组成型表达,可产生对流感病毒的选择性抵抗力。  |  Staeheli, P., et al. 1986. Cell. 44: 147-58. PMID: 3000619

订购信息

产品名称产品编号规格价格数量收藏夹

Mx1 抗体 (E-5)

sc-271024
200 µg/ml
$322.00

Mx1 (E-5): m-IgG Fc BP-HRP 套装

sc-529099
200 µg Ab; 10 µg BP
$361.00

Mx1 (E-5): m-IgGκ BP-HRP 套装

sc-521818
200 µg Ab, 40 µg BP
$361.00

Mx1 抗体 (E-5) AC

sc-271024 AC
500 µg/ml, 25% agarose
$424.00

Mx1 抗体 (E-5) HRP

sc-271024 HRP
200 µg/ml
$322.00

Mx1 抗体 (E-5) FITC

sc-271024 FITC
200 µg/ml
$336.00

Mx1 抗体 (E-5) PE

sc-271024 PE
200 µg/ml
$349.00

Mx1 抗体 (E-5) Alexa Fluor® 488

sc-271024 AF488
200 µg/ml
$364.00

Mx1 抗体 (E-5) Alexa Fluor® 546

sc-271024 AF546
200 µg/ml
$364.00

Mx1 抗体 (E-5) Alexa Fluor® 594

sc-271024 AF594
200 µg/ml
$364.00

Mx1 抗体 (E-5) Alexa Fluor® 647

sc-271024 AF647
200 µg/ml
$364.00

Mx1 抗体 (E-5) Alexa Fluor® 680

sc-271024 AF680
200 µg/ml
$364.00

Mx1 抗体 (E-5) Alexa Fluor® 790

sc-271024 AF790
200 µg/ml
$364.00

Has the use of this Ab in IHC for mouse tissue been published or reported anywhere?

Asked by: Zulma26
Thank you for your question. We are not currently aware of any product citations using this antibody for IHC in mouse tissue. We do recommend and warranty this antibody for IHC in fresh or frozen mouse tissue fixed in acetone or methanol.
Answered by: Technical Support
Date published: 2019-04-13

I need to use an antibody to detect the protein in samples of monkey origin. Do you know if sc-271024: Mx1 (E-5) mouse monoclonal antibody will work?

Asked by: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-03-06
  • y_2026, m_2, d_6, h_6CST
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Rated 4 out of 5 by from WB human cells good, not working in canine cellsProminent band at roughly 70 kDa in Western blot on IFN-stimulated or virus-infected human cells; no endogenous Mx1 detected in resting cells. Doesn't work in canine cells.
Date published: 2020-10-01
Rated 4 out of 5 by from good antibodyMx1 Antibody (E-5) did work by Western Blot of A549 (1:1000 overnight) in sev infected cells but the band was weak in uninfected cells
Date published: 2017-09-15
Rated 1 out of 5 by from does not work on macaque tissuethis antibody did not work by IF of paraffin embedded retina tissue (1:50 overnight) or by immunoblot (1:200 overnight) on macaque retina lysates
Date published: 2017-06-08
Rated 5 out of 5 by from Good Western Blot data of Mx1 expression in nonGood Western Blot data of Mx1 expression in non-transfected and human Mx1 transfected 293T whole cell lysates. -SCBT QC
Date published: 2013-07-06
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Mx1 抗体 (E-5) is rated 3.5 out of 5 by 4.
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