Mx1/2/3 Antibody (C-1): sc-166412

Mx1/2/3 Antibody (C-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects Mx1/2/3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Mx1/2/3 (C-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Mx1/2/3 proteins, particularly Mx1 and Mx2, play crucial roles in immune response to viral infections, functioning as GTPases within the Dynamin family of microtubule-associated proteins. Mx2, localized in the cytoplasm, is induced by interferon-alpha and interferon-beta, leading to accumulation and subsequent inhibition of vesicular stomatitis virus replication, providing resistance to viral infection. Mx1 is found in the nucleus and, upon similar induction, offers selective resistance against highly pathogenic H5N1 influenza virus. Mx3 serves as a rat-specific member of this protein family, contributing to overall antiviral defense mechanisms. Mx1 and Mx2 localization to different cellular compartments underscores distinct yet complementary roles in immune response, making anti-Mx1/2/3 antibody (C-1) an essential tool for studying viral resistance mechanisms in various species.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Mx1/2/3 Antibody (C-1) References:

1. Identification of the murine Mx2 gene: interferon-induced expression of the Mx2 protein from the feral mouse gene confers resistance to vesicular stomatitis virus.  |  Jin, HK., et al. 1999. J Virol. 73: 4925-30. PMID: 10233954
2. Functional diversity of Mx proteins: variations on a theme of host resistance to infection.  |  Lee, SH. and Vidal, SM. 2002. Genome Res. 12: 527-30. PMID: 11932237
3. Mouse Mx2 protein inhibits vesicular stomatitis virus but not influenza virus.  |  Zürcher, T., et al. 1992. Virology. 187: 796-800. PMID: 1312277
4. INHERITANCE OF RESISTANCE TO INFLUENZA VIRUS IN MICE.  |  LINDENMANN, J. 1964. Proc Soc Exp Biol Med. 116: 506-9. PMID: 14193387
5. The Mx1 gene protects mice against the pandemic 1918 and highly lethal human H5N1 influenza viruses.  |  Tumpey, TM., et al. 2007. J Virol. 81: 10818-21. PMID: 17652381
6. Mx1 gene protects mice against the highly lethal human H5N1 influenza virus.  |  Salomon, R., et al. 2007. Cell Cycle. 6: 2417-21. PMID: 17700072
7. Identification of a second interferon-regulated murine Mx gene.  |  Staeheli, P. and Sutcliffe, JG. 1988. Mol Cell Biol. 8: 4524-8. PMID: 2460745
8. Organization of the murine Mx gene and characterization of its interferon- and virus-inducible promoter.  |  Hug, H., et al. 1988. Mol Cell Biol. 8: 3065-79. PMID: 2974922
9. Mx protein: constitutive expression in 3T3 cells transformed with cloned Mx cDNA confers selective resistance to influenza virus.  |  Staeheli, P., et al. 1986. Cell. 44: 147-58. PMID: 3000619
10. Rare variant MX1 alleles increase human susceptibility to zoonotic H7N9 influenza virus.  |  Chen, Y., et al. 2021. Science. 373: 918-922. PMID: 34413236
11. MX2 Viral Substrate Breadth and Inhibitory Activity Are Regulated by Protein Phosphorylation.  |  Betancor, G., et al. 2022. mBio. 13: e0171422. PMID: 35880880
12. MX2 forms nucleoporin-comprising cytoplasmic biomolecular condensates that lure viral capsids.  |  Moschonas, GD., et al. 2024. Cell Host Microbe. 32: 1705-1724.e14. PMID: 39389033

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Mx1/2/3 Antibody (C-1) has 25 citations in a variety of scientific publications.

Primary antibodies like Anti-Mx1/2/3 Antibody (C-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Mx1/2/3 Antibody (C-1) Specifications

Alternate Product Name: Mx1/2/3

Product Clone Name: C-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 47-73

Epitope Target: Mx1

Epitope Species: human

Product Reactivity/Detection: Mx1 and Mx2 of human and mouse origin, and Mx1, Mx2 and Mx3 of rat origin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine and bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 25 citations in various scientific publications

Available Conjugates/Forms for Mx1/2/3 Antibody (C-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin