MUS81 Antibody (MTA30 2G10/3): sc-53382
- MUS81 Antibody (MTA30 2G10/3) is a mouse monoclonal IgG2a κ, cited in 26 publications, provided at 200 µg/ml
- raised against Mus81 of human origin
- recommended for detection of MUS81 of human origin by WB and IP
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MUS81 Antibody (MTA30 2G10/3) for WB applications. These reagents are now offered in bundles with MUS81 Antibody (MTA30 2G10/3) (see ordering information below).
QUICK LINKS
SEE ALSO...
MUS81 Antibody (MTA30 2G10/3) is a mouse monoclonal IgG2a antibody that detects MUS81 in human samples through western blotting (WB) and immunoprecipitation (IP) applications. MUS81 plays a crucial role in maintaining genomic integrity by participating in the DNA damage response, particularly in the repair of DNA lesions induced by UV radiation and methylation. MUS81 is essential for the resolution of stalled replication forks, as MUS81 forms a complex with Eme1 to create an endonuclease that processes these forks before they can regress into Holliday junctions. MUS81′s ability to resolve Holliday junctions into linear duplexes by cleaving strands of like polarity is vital for preventing genomic instability, which can lead to cancer. Additionally, MUS81 expression is upregulated in response to agents that inhibit DNA replication, highlighting MUS81′s importance in cellular stress responses. The human MUS81 gene is located on chromosome 11q13 and encodes a protein consisting of 551 amino acids, underscoring MUS81′s significance in cellular mechanisms that safeguard the genome against damage.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MUS81 Antibody (MTA30 2G10/3) References:
- Human Mus81-associated endonuclease cleaves Holliday junctions in vitro. | Chen, XB., et al. 2001. Mol Cell. 8: 1117-27. PMID: 11741546
- The MUS81 endonuclease is essential for telomerase negative cell proliferation. | Zeng, S. and Yang, Q. 2009. Cell Cycle. 8: 2157-60. PMID: 19617716
- The DNA repair endonuclease Mus81 facilitates fast DNA replication in the absence of exogenous damage. | Fu, H., et al. 2015. Nat Commun. 6: 6746. PMID: 25879486
- SLX4-SLX1 Protein-independent Down-regulation of MUS81-EME1 Protein by HIV-1 Viral Protein R (Vpr). | Zhou, X., et al. 2016. J Biol Chem. 291: 16936-16947. PMID: 27354282
- STC2 as a novel mediator for Mus81-dependent proliferation and survival in hepatocellular carcinoma. | Wu, F., et al. 2017. Cancer Lett. 388: 177-186. PMID: 27939696
- Mus81 knockdown sensitizes colon cancer cells to chemotherapeutic drugs by activating CHK1 pathway. | Wu, F., et al. 2017. Clin Res Hepatol Gastroenterol. 41: 592-601. PMID: 28291626
- MUS81 nuclease activity is essential for replication stress tolerance and chromosome segregation in BRCA2-deficient cells. | Lai, X., et al. 2017. Nat Commun. 8: 15983. PMID: 28714477
- Mus81-Eme1-dependent aberrant processing of DNA replication intermediates in mitosis impairs genome integrity. | Calzetta, NL., et al. 2020. Sci Adv. 6: PMID: 33298441
- A protocol to determine the activities of human MUS81-EME1&2 endonucleases. | Fang, Q., et al. 2022. STAR Protoc. 3: 101528. PMID: 35819885
- Downregulation of MUS81 expression inhibits cell migration and maintains EBV latent infection through miR-BART9-5p in EBV-associated gastric cancer. | Zhang, Y., et al. 2023. J Med Virol. 95: e28725. PMID: 37185865
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MUS81 Antibody (MTA30 2G10/3) | sc-53382 | 200 µg/ml | $322.00 | |||
MUS81 Antibody (MTA30 2G10/3): m-IgG Fc BP-HRP Bundle | sc-536949 | 200 µg Ab; 10 µg BP | $361.00 | |||
MUS81 Antibody (MTA30 2G10/3): m-IgGκ BP-HRP Bundle | sc-534126 | 200 µg Ab; 40 µg BP | $361.00 | |||
MUS81 Antibody (MTA30 2G10/3): m-IgG2a BP-HRP Bundle | sc-547907 | 200 µg Ab; 10 µg BP | $361.00 |