MUS81 Antibody (2G10/3): sc-47692

MUS81 Antibody (2G10/3) is a mouse monoclonal IgG2a kappa light chain antibody that detects the MUS81 protein of human origin by western blotting (WB) and immunoprecipitation (IP). MUS81 (2G10/3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MUS81 plays a critical role in DNA repair mechanisms, particularly in response to DNA damage caused by UV radiation and methylation, which is essential for maintaining genomic integrity. Located in the nucleus, MUS81 is part of a complex that includes Eme1, forming an endonuclease that processes stalled replication forks, preventing the formation of potentially harmful structures such as Holliday junctions. This processing is vital for ensuring that DNA replication can continue smoothly, especially under conditions of replicational stress, such as those induced by genotoxic agents. The gene encoding human MUS81 is situated on chromosome 11q13 and produces a 551 amino acid protein that is upregulated in response to DNA replication blockers, highlighting its importance in cellular responses to DNA damage. Anti-MUS81 antibody (2G10/3) enables researchers to gain insights into the mechanisms of DNA repair and the cellular responses to genotoxic stress, making this antibody a valuable tool in the study of cancer biology and therapeutic interventions.

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MUS81 Antibody (2G10/3) References:

1. MUS81 encodes a novel helix-hairpin-helix protein involved in the response to UV- and methylation-induced DNA damage in Saccharomyces cerevisiae.  |  Interthal, H. and Heyer, WD. 2000. Mol Gen Genet. 263: 812-27. PMID: 10905349
2. Damage tolerance protein Mus81 associates with the FHA1 domain of checkpoint kinase Cds1.  |  Boddy, MN., et al. 2000. Mol Cell Biol. 20: 8758-66. PMID: 11073977
3. Human Mus81-associated endonuclease cleaves Holliday junctions in vitro.  |  Chen, XB., et al. 2001. Mol Cell. 8: 1117-27. PMID: 11741546
4. Mus81-Eme1 and Rqh1 involvement in processing stalled and collapsed replication forks.  |  Doe, CL., et al. 2002. J Biol Chem. 277: 32753-9. PMID: 12084712
5. Rad54 and Mus81 cooperation promotes DNA damage repair and restrains chromosome missegregation.  |  Ghamrasni, SE., et al. 2016. Oncogene. 35: 4836-45. PMID: 26876210
6. MUS81 is associated with cell proliferation and cisplatin sensitivity in serous ovarian cancer.  |  Xie, S., et al. 2016. Biochem Biophys Res Commun. 476: 493-500. PMID: 27255997
7. Phosphorylation by CK2 regulates MUS81/EME1 in mitosis and after replication stress.  |  Palma, A., et al. 2018. Nucleic Acids Res. 46: 5109-5124. PMID: 29850896
8. Targeting MUS81 promotes the anticancer effect of WEE1 inhibitor and immune checkpoint blocking combination therapy via activating cGAS/STING signaling in gastric cancer cells.  |  Li, C., et al. 2021. J Exp Clin Cancer Res. 40: 315. PMID: 34625086
9. Expression of MUS81 Mediates the Sensitivity of Castration-Resistant Prostate Cancer to Olaparib.  |  Gong, L., et al. 2022. J Immunol Res. 2022: 4065580. PMID: 35910852
10. Identification of small-molecule inhibitors of human MUS81-EME1/2 by FRET-based high-throughput screening.  |  Zhang, X., et al. 2023. Bioorg Med Chem. 90: 117383. PMID: 37352577
11. TDP1 phosphorylation by CDK1 in mitosis promotes MUS81-dependent repair of trapped Top1-DNA covalent complexes.  |  Paul Chowdhuri, S. and Das, BB. 2024. EMBO J. 43: 3710-3732. PMID: 39014228
12. Senataxin RNA/DNA helicase promotes replication restart at co-transcriptional R-loops to prevent MUS81-dependent fork degradation.  |  Rao, S., et al. 2024. Nucleic Acids Res. 52: 10355-10369. PMID: 39119900

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MUS81 Antibody (2G10/3) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-MUS81 Antibody (2G10/3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MUS81 Antibody (2G10/3) Specifications

Alternate Product Name: MUS81

Product Clone Name: 2G10/3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: MUS81

Epitope Species: human

Product Reactivity/Detection: MUS81

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for MUS81 Antibody (2G10/3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin