MTA1 Antibody (A-11): sc-17773

MTA1 Antibody (A-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects MTA1 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHCP), and enzyme-linked immunosorbent assay (ELISA). MTA1 Antibody (A-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. MTA1, or metastasis-associated protein 1, plays a crucial role as a component of the NURD (nucleosome remodeling and histone deacetylation) complex, which is essential for ATP-dependent chromatin remodeling and histone deacetylase activity. MTA1 is primarily located in the nucleus, where MTA1 facilitates transcriptional repression by promoting the association of repressor molecules with chromatin, thereby influencing gene expression. The structural integrity of MTA1, which includes a single SH3-binding motif and a zinc finger domain, is vital for function, as these domains are involved in protein-protein interactions that are critical for the assembly and activity of the NURD complex. MTA1 is typically expressed at low levels across various tissues but shows significantly higher expression in the testis. MTA1 overexpression has been linked to tumor invasion and metastasis in several carcinomas, including colorectal, gastrointestinal, and breast cancers, suggesting that MTA1 levels may serve as a potential biomarker for assessing tumor malignancy. MTA1 shares over 55% sequence homology with MTA2 (MTA1-like protein 1), which is ubiquitously expressed and may also play a role in cancer progression.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

MTA1 Antibody (A-11) References:

1. Role of MTA1 in cancer progression and metastasis.  |  Sen, N., et al. 2014. Cancer Metastasis Rev. 33: 879-89. PMID: 25344802
2. Towards an understanding of the structure and function of MTA1.  |  Millard, CJ., et al. 2014. Cancer Metastasis Rev. 33: 857-67. PMID: 25352341
3. MTA1, a metastasis‑associated protein, in endothelial cells is an essential molecule for angiogenesis.  |  Ishikawa, M., et al. 2022. Mol Med Rep. 25: PMID: 34779499
4. RUNX2 recruits the NuRD(MTA1)/CRL4B complex to promote breast cancer progression and bone metastasis.  |  Yin, X., et al. 2022. Cell Death Differ. 29: 2203-2217. PMID: 35534547
5. MTA1, a Novel ATP Synthase Complex Modulator, Enhances Colon Cancer Liver Metastasis by Driving Mitochondrial Metabolism Reprogramming.  |  Wang, T., et al. 2023. Adv Sci (Weinh). 10: e2300756. PMID: 37442756
6. Analysis of the complete sequence of the novel metastasis-associated candidate gene, mta1, differentially expressed in mammary adenocarcinoma and breast cancer cell lines.  |  Toh, Y., et al. 1995. Gene. 159: 97-104. PMID: 7607577
7. A novel candidate metastasis-associated gene, mta1, differentially expressed in highly metastatic mammary adenocarcinoma cell lines. cDNA cloning, expression, and protein analyses.  |  Toh, Y., et al. 1994. J Biol Chem. 269: 22958-63. PMID: 8083195
8. A complex containing N-CoR, mSin3 and histone deacetylase mediates transcriptional repression.  |  Heinzel, T., et al. 1997. Nature. 387: 43-8. PMID: 9139820
9. Overexpression of the MTA1 gene in gastrointestinal carcinomas: correlation with invasion and metastasis.  |  Toh, Y., et al. 1997. Int J Cancer. 74: 459-63. PMID: 9291440
10. cDNA cloning of a novel, developmentally regulated immediate early gene activated by fibroblast growth factor and encoding a nuclear protein.  |  Paterno, GD., et al. 1997. J Biol Chem. 272: 25591-5. PMID: 9325278
11. NURD, a novel complex with both ATP-dependent chromatin-remodeling and histone deacetylase activities.  |  Xue, Y., et al. 1998. Mol Cell. 2: 851-61. PMID: 9885572
12. Molecular cloning, mapping, and characterization of a novel human gene, MTA1-L1, showing homology to a metastasis-associated gene, MTA1.  |  Futamura, M., et al. 1999. J Hum Genet. 44: 52-6. PMID: 9929979

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MTA1 Antibody (A-11) has 46 citations in a variety of scientific publications.

Primary antibodies like Anti-MTA1 Antibody (A-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MTA1 Antibody (A-11) Specifications

Alternate Product Name: Metastasis associated 1

Product Clone Name: A-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 513-678

Epitope Target: MTA1

Epitope Species: human

Product Reactivity/Detection: MTA1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 46 citations in various scientific publications

Available Conjugates/Forms for MTA1 Antibody (A-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent

The preferred MTA1 antibody is MTA1 Antibody (E-12): sc-373765.