Mpk1 Antibody (H-2) recommended for detection of Mpk1 of S. cerevisiae origin by WB, IP, IF and ELISA

Mpk1 Antibody (H-2): sc-165979

Mpk1 Antibody (H-2) is rated 5.0 out of 5 by 1.
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Datasheets

    • Mpk1 Antibody (H-2) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 241-484 of Mpk1 of Saccharomyces cerevisiae origin
    • recommended for detection of Mpk1 of S. cerevisiae origin by WB, IP, IF and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.

How much sample do you recommend loading on the gel for Western blot detection using Mpk1 (H-2): sc-165979?

Asked by: cjMara
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Good Western blot data of yeast recombinant Good Western blot data of yeast recombinant Mpk1 fusion protein. -SCBT QC
Date published: 2013-08-27
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