Mpk1 Antibody (H-2): sc-165979

Mpk1 Antibody (H-2) is a mouse monoclonal IgG2b antibody that detects Mpk1 in Saccharomyces cerevisiae through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Mpk1, also known as Slt2, is a crucial component of the MAP kinase signaling cascade that plays a vital role in maintaining cell wall integrity in yeast. Mpk1 is primarily located in the cytoplasm and is activated in response to cell wall stress, which is essential for yeast′s ability to adapt to environmental changes and maintain cellular structure. Mpk1 function is critical, as mutants lacking Mpk1 exhibit severe defects in cell lysis due to compromised cell wall synthesis, leading to impaired growth and survival. Additionally, Mpk1 interacts with various upstream kinases, including Bck1 and Mkk1/Mkk2, which are integral to the signaling pathway that regulates cell wall integrity. Anti-Mpk1 antibody (H-2) provides a reliable tool for researchers studying the dynamics of this important signaling pathway and its implications in yeast biology.

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Mpk1 Antibody (H-2) References:

1. Saccharomyces cerevisiae mid2p is a potential cell wall stress sensor and upstream activator of the PKC1-MPK1 cell integrity pathway.  |  Ketela, T., et al. 1999. J Bacteriol. 181: 3330-40. PMID: 10348843
2. A synthetic analysis of the Saccharomyces cerevisiae stress sensor Mid2p, and identification of a Mid2p-interacting protein, Zeo1p, that modulates the PKC1-MPK1 cell integrity pathway.  |  Green, R., et al. 2003. Microbiology (Reading). 149: 2487-2499. PMID: 12949174
3. Yeast Mpk1 mitogen-activated protein kinase activates transcription through Swi4/Swi6 by a noncatalytic mechanism that requires upstream signal.  |  Kim, KY., et al. 2008. Mol Cell Biol. 28: 2579-89. PMID: 18268013
4. Slt2 (Mpk1) MAP kinase is involved in the response of Saccharomyces cerevisiae to 8-methoxypsoralen plus UVA.  |  Dardalhon, M., et al. 2009. J Photochem Photobiol B. 95: 148-55. PMID: 19318276
5. Mechanism of Mpk1 mitogen-activated protein kinase binding to the Swi4 transcription factor and its regulation by a novel caffeine-induced phosphorylation.  |  Truman, AW., et al. 2009. Mol Cell Biol. 29: 6449-61. PMID: 19805511
6. Variants of the yeast MAPK Mpk1 are fully functional independently of activation loop phosphorylation.  |  Goshen-Lago, T., et al. 2016. Mol Biol Cell. 27: 2771-83. PMID: 27413009
7. Phosphatidylinositol 3,5-bisphosphate is involved in methylglyoxal-induced activation of the Mpk1 mitogen-activated protein kinase cascade in Saccharomyces cerevisiae.  |  Nomura, W., et al. 2017. J Biol Chem. 292: 15039-15048. PMID: 28743744
8. The MAPK Slt2/Mpk1 plays a role in iron homeostasis through direct regulation of the transcription factor Aft1.  |  Pujol-Carrion, N., et al. 2021. Biochim Biophys Acta Mol Cell Res. 1868: 118974. PMID: 33549702
9. The SLT2 (MPK1) MAP kinase homolog is involved in polarized cell growth in Saccharomyces cerevisiae.  |  Mazzoni, C., et al. 1993. J Cell Biol. 123: 1821-33. PMID: 8276900
10. Characterization of a serum response factor-like protein in Saccharomyces cerevisiae, Rlm1, which has transcriptional activity regulated by the Mpk1 (Slt2) mitogen-activated protein kinase pathway.  |  Watanabe, Y., et al. 1997. Mol Cell Biol. 17: 2615-23. PMID: 9111331

The preferred Mpk1 antibody is Mpk1 Antibody (D-1): sc-374434.