Anti-Mpk1 Antibody (D-1) is a mouse monoclonal IgG1 (kappa light chain) Mpk1 antibody provided at 200 µg/ml
specific for an epitope mapping between amino acids 453-491 near the C-terminus of Mpk1 of Saccharomyces cerevisiae origin
Mpk1 Antibody (D-1) is recommended for detection of Mpk1 of y origin by WB, IP, IF and ELISA
Anti-Mpk1 Antibody (D-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
blocking peptide, sc-374434 P
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Mpk1 (D-1): sc-374434.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
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Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Mpk1 Antibody (D-1) is a high quality monoclonal Mpk1 antibody (also designated Mpk1 antibody) suitable for the detection of the Mpk1 protein of Saccharomyces cerevisiae origin. Mpk1 Antibody (D-1) is available as both the non-conjugated anti-Mpk1 antibody form, as well as multiple conjugated forms of anti-Mpk1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Yeasts maintain the integrity of their cell walls via a MAP kinase cascade. This cascade consists of a MAP kinase (mitogen-activated protein kinase, also called ERK, for extracellular regulated kinase) as well as several upstream regulatory kinases (MAPKKs or MEKs, for MAP/ERK kinase). Pkc1 (also designated Sst1), a yeast homolog of the mammalian PKC a, b and g isoforms, transmits extracellular signals to Bck1, a MAPKKK (also called Slk1, Ssp31 or Las3). Bck1 then activates two MAPKKs, Mkk1 and Mkk2 (also referred to as Ssp32 and Ssp33, respectively). These in turn activate the MAP kinase Mpk1 (also called Slt2). Mutants lacking any component of this cascade exhibit a defect in cell lysis resulting from deficient cell wall synthesis. Bck2 (also designated Ctr7) has been identified as a suppressor of Pkc1 and Mpk1 deletions.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
What application is the blocking peptide sc-374434 P appropriate for?
Asked by: jenni
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here:
Rated 5 out of
Works great in total yeast lysateGave a nice band at the expected size with TCA precipitated whole cell yeast (S. cerevisiae) lysate. Upregulation seen in the expected samples. Worked great. Very clean.
Date published: 2017-11-08
Rated 5 out of
Produced nice Western blot data of yeastProduced nice Western blot data of yeast recombinant Mpk1 fusion protein and expression in S. cerevisiae whole cell lysate . -SCBT QC
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