Mpk1 Antibody (E-9) recommended for detection of Mpk1 of S. cerevisiae origin by WB, IP, IF and ELISA

Mpk1 Antibody (E-9): sc-133189

Mpk1 Antibody (E-9) is rated 5.0 out of 5 by 1.
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Datasheets

    • Mpk1 Antibody (E-9) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 241-484 of Mpk1 of Saccharomyces cerevisiae origin
    • recommended for detection of Mpk1 of S. cerevisiae origin by WB, IP, IF and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.

What blocking solution should I use with Mpk1 (E-9): sc-133189 monoclonal antibody for Western blot?

Asked by: Cweed
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-04-12
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Rated 5 out of 5 by from Good Western blot data of yeast recombinant Good Western blot data of yeast recombinant Mpk1 under reducing conditions. -SCBT QC
Date published: 2014-11-21
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