Mpk1 Antibody (E-9): sc-133189

Mpk1 Antibody (E-9) is a mouse monoclonal IgG1 antibody that detects Mpk1 in Saccharomyces cerevisiae through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Mpk1, also known as Slt2, functions as a crucial component of the MAP kinase signaling cascade, playing a vital role in maintaining cell wall integrity in yeast. Mpk1 primarily localizes in the cytoplasm and translocates to the nucleus upon activation, where Mpk1 regulates gene expression in response to cell wall stress. Proper functioning of Mpk1 remains essential for yeast′s ability to withstand environmental stresses and maintain cellular structure, as mutants lacking Mpk1 exhibit severe defects in cell wall synthesis and increased susceptibility to lysis. Mpk1 participates in various cellular processes, including cell cycle regulation and apoptosis, making Mpk1 a key player in overall yeast cell health and viability. anti-Mpk1 antibody (E-9) serves as an invaluable tool for researchers studying the MAP kinase pathway and cellular responses to stress, providing insights into fundamental mechanisms of yeast biology.

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Mpk1 Antibody (E-9) References:

1. Cell wall perturbation in yeast results in dual phosphorylation of the Slt2/Mpk1 MAP kinase and in an Slt2-mediated increase in FKS2-lacZ expression, glucanase resistance and thermotolerance.  |  de Nobel, H., et al. 2000. Microbiology (Reading). 146 (Pt 9): 2121-2132. PMID: 10974100
2. Response of the Saccharomyces cerevisiae Mpk1 mitogen-activated protein kinase pathway to increases in internal turgor pressure caused by loss of Ppz protein phosphatases.  |  Merchan, S., et al. 2004. Eukaryot Cell. 3: 100-7. PMID: 14871941
3. Mpk1 MAPK association with the Paf1 complex blocks Sen1-mediated premature transcription termination.  |  Kim, KY. and Levin, DE. 2011. Cell. 144: 745-56. PMID: 21376235
4. TORC1 controls G1-S cell cycle transition in yeast via Mpk1 and the greatwall kinase pathway.  |  Moreno-Torres, M., et al. 2015. Nat Commun. 6: 8256. PMID: 26356805
5. TORC1, Tel1/Mec1, and Mpk1 regulate autophagy induction after DNA damage in budding yeast.  |  Ueda, S., et al. 2019. Cell Signal. 62: 109344. PMID: 31201849
6. Crosstalk between Saccharomycescerevisiae SAPKs Hog1 and Mpk1 is mediated by glycerol accumulation.  |  Laz, EV., et al. 2020. Fungal Biol. 124: 361-367. PMID: 32389298
7. Hsp90 and phosphorylation of the Slt2(Mpk1) MAP kinase activation loop are essential for catalytic, but not non-catalytic, Slt2-mediated transcription in yeast.  |  Millson, SH., et al. 2022. Cell Stress Chaperones. 27: 295-304. PMID: 35420390
8. Characterization of domains in the yeast MAP kinase Slt2 (Mpk1) required for functional activity and in vivo interaction with protein kinases Mkk1 and Mkk2.  |  Soler, M., et al. 1995. Mol Microbiol. 17: 833-42. PMID: 8596433
9. Genetic evidence for the functional redundancy of the calcineurin- and Mpk1-mediated pathways in the regulation of cellular events important for growth in Saccharomyces cerevisiae.  |  Nakamura, T., et al. 1996. Mol Gen Genet. 251: 211-9. PMID: 8668132
10. Role of calcineurin and Mpk1 in regulating the onset of mitosis in budding yeast.  |  Mizunuma, M., et al. 1998. Nature. 392: 303-6. PMID: 9521328

The preferred Mpk1 antibody is Mpk1 Antibody (D-1): sc-374434.